Bioconversion Process for Producing Nylon-7, Nylon-7, 7 and Polyesters
AssigneeInvista Technologies S.A R.L.
InventorsPaul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie, Benjamin D. Herzog
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Abstract
Embodiments of the present invention relate to methods for the biosynthesis of di- or trifunctional C7 alkanes in the presence of isolated enzymes or in the presence of a recombinant host cell expressing those enzymes. The di- or trifunctional C7 alkanes are useful as intermediates in the production of nylon-7, nylon-7,x, nylon-x,7, and polyesters.
Claims36
1-187. (canceled)
A method of converting a compound, the method comprising contacting pimeloyl-CoA (PCoA) or pimeloyl [acp] (PACP) with an enzyme that catalyzes the thioester hydrolysis of PCoA or PACP to pimelic acid (PA), wherein PA is produced.
A method of converting a compound, the method comprising contacting PCoA or PACP with an enzyme that catalyzes the reduction of PCoA or PACP to PAS, wherein PAS is produced.
A method of converting a compound, the method comprising contacting α-ketosuberate (AKS) with an enzyme that catalyzes the α-keto decarboxylation of AKS to PAS, wherein PAS is produced.
A method of converting a compound, the method comprising contacting AKS with an enzyme that catalyzes the transfer of an amino group to the AKS to produce α-amino suberate (AAS), wherein AAS is produced.
A method of converting a compound, the method comprising contacting PAS with an enzyme that catalyzes the semialdehyde amination of the PAS to 7-amino-heptanoic acid (7 AHA), wherein 7 AHA is produced.
A method of converting a compound, the method comprising contacting 7 AHT with an enzyme that catalyzes the transfer of an amino group to the 7 AHT to produce 1,7-diaminoheptane (1,7 DAH), wherein 1,7 DAH is produced.
The method of claim 288, wherein the enzyme, or one or more enzymes, is or are:
- (i) isolated;
- (ii) in a cell lysate or partially purified cell lysate;
- or (iii) in a host cell recombinantly expressing it or them.
An isolated cell comprising one or more exogenous nucleic acid encoding:
- (a) (i) a thioesterase, an acid thiol ligase, or a CoA transferase;
- and (ii) a carboxylic acid reductase;
- (b) (i) a fatty-acyl-CoA reductase or an aldehyde dehydrogenase;
- and (ii) an enzyme that catalyzes a reaction whose product is PCoA or PACP;
- (c) (i) a keto acid decarboxylase or an acetolactate synthase;
- and (ii) and enzyme that catalyzes a reaction whose product is AKS;
- (d) (i) an aminotransferase;
- and (ii) a decarboxylase or an acetolactate synthase;
- or
- (e) (i) an aminotransferase or a ω-transaminase;
- and (ii) an enzyme that catalyzes a reaction whose product is 7 AHT.
Dependencies are inferred from the claim text.