Plant Transformation Method

A method for producing a transgenic plant, comprising the steps of:

• (i) providing a wounded transformable explant, comprising at least one cotyledon, and wounded meristematic tissue of a primary or higher leaf node;
• (ii) transforming cells comprised by the said explant with a polynucleotide comprising at least one plant expression cassette for a selectable marker gene;
• (iii) transferring said explant to a shoot induction medium and cultivating said explant on said shoot induction medium comprising at least one selection compound for said selectable marker gene, thereby allowing formation of at least one de novo formed shoot comprising plant cells comprising said least one plant expression cassette for said selectable marker gene;
• (iv) separating the meristem region of said primary or higher leaf node comprising said at least one de novo formed shoot and transferring said meristem region to a hydroponic medium, said hydroponic medium comprising at least one selection compound for said selectable marker gene, and allowing said at least one de novo formed shoot to elongate;
• and
• (v) regenerating a transgenic plant from said plantlet.