SPL16 compositions and methods to increase agronomic performance of plants
AssigneeInstitute of Genetics and Development Biology
InventorsXiangdong Fu, Shaokui Wang, Kun Wu, Guiquan Zhang, Ruizhen Zeng
Life of this patent
Estimating the term…
Abstract
Methods and compositions that affect grain shape, width, and yield in rice are disclosed. Methods of transgenic modulation and marker-assisted breeding methods improve grain length to width ratio and grain yield in rice. Down-regulation of OsSPL16 in rice resulted in increased grain quality.
Claims23
A method of improving grain width, weight or yield of a plant, the method comprising modulating the expression of (i) a polynucleotide encoding an amino acid sequence comprising one of SEQ ID NOS:
- 9-11 or an amino acid sequence that is at least 95% identical to one of SEQ ID NOS:
- 9-11 (ii) a polynucleotide that hybridizes under stringent hybridization conditions to a fragment of polynucleotide selected from the group consisting of SEQ ID NOS:
- 1-6, wherein the fragment comprises at least 100 contiguous nucleotides of SEQ ID NOS:
- 1-6 (iii) a polynucleotide that encodes an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 9, (iv) a polynucleotide encoding a polypeptide comprising one or more deletions or insertions or substitution of amino acids compared to SEQ ID NO:
- 9, wherein the polynucleotide encodes a polypeptide involved in the regulation of grain width, weight or yield.
A method of improving the length to width aspect of rice grain, the method comprising modulating the expression of a polynucleotide that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 9 or an allelic variant thereof.
A method of improving rice grain yield, the method comprising the expression of a polynucleotide that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 9 or a variant thereof.
A method of marker assisted selection of a plant for improved grain quality or yield, the method comprising:
- a. performing marker-assisted selection of plants that have one or more variations in genomic regions encoding a protein comprising SEQ ID NO:
- 9 or a variant thereof or a regulatory sequence thereof;
- and
- b. identifying the plant that produces grains with improved grain quality and/or higher yield.
A method of identifying alleles in rice plants or rice germplasm that are associated with improved grain quality and/or increased yield, the method comprising:
- a. obtaining a population of rice plants, wherein one or more plants exhibit improved grain quality and/or increased yield;
- b. evaluating allelic variations with respect to the polynucleotide sequence encoding a protein comprising a polypeptide comprising:
- SEQ ID NO:
- 9 or in the genomic region that regulates the expression of the polynucleotide encoding the protein;
- c. obtaining phenotypic values of improved grain quality and/or increased yield for a plurality of rice plants in the population;
- d. associating the allelic variations in the genomic region associated with the polynucleotide with the phenotype;
- and
- e. identifying the alleles that are associated with improved grain quality and/or increased yield.
An isolated polynucleotide (i) encoding an amino acid sequence comprising one of SEQ ID NOS:
- 9-11 or an amino acid sequence that is at least 95% identical to one of SEQ ID NOS:
- 9-11 (ii) hybridizing under stringent hybridization conditions to a fragment of polynucleotide selected from the group consisting of SEQ ID NOS:
- 1-6, wherein the fragment comprises at least 100 contiguous nucleotides of SEQ ID NOS:
- 1-6 (iii) that encodes an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 9, (iv) a polynucleotide encoding a polypeptide comprising one or more deletions or insertions or substitution of amino acids compared to SEQ ID NO:
- 9, wherein the polynucleotide encodes a polypeptide involved in the regulation of grain width, weight or yield.
An isolated polynucleotide that operably regulates the expression of a polynucleotide that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 9 or a variant thereof.
Dependencies are inferred from the claim text.