Genetic loci associated with fusarium ear mold resistance in maize
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsSTANLEY LUCK, Dario Prada, Kevin Simcox, Adriana Tomas
Life of this patent
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Abstract
The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to Fusarium ear mold. Maize plants generated by the methods of the invention are also a feature of the invention.
Claims13
A method of selecting a maize plant with enhanced resistance to Fusarium ear mold comprising:
- a. obtaining DNA accessible for analysis;
- b. detecting in the maize plant a first marker allele that is linked to and associated with a second marker allele selected from the group consisting of:
- i.
- xiii. a “G” at PHM9362-8,
- xiv. a “G” at PHM1147-16,
- xv. a “T” at PHM11850-3,
- xvi. a “C” at PHM11850-6,
- xvii. an “A” at PHM13773-6,
- xviii. a “C” at PHM13773-11,
- xix. an “A” at PHM16422-11,
- xx. a “T” at PHM1147-19,
- xxi. a “G” at PHM5280-41,
- xxii. a “T” at PHM9301-37, and
- xxiii. a “T” at PHM4423-4;
- and
- c. selecting said maize plant that has the first marker allele.
A method of selecting a maize plant with enhanced Fusarium ear mold resistance comprising:
- a. obtaining DNA accessible for analysis;
- b. detecting in the maize plant at least one marker allele selected from the group consisting of:
- i.
- xiii. a “G” at PHM9362-8,
- xiv. a “G” at PHM1147-16,
- xv. a “T” at PHM11850-3,
- xvi. a “C” at PHM11850-6,
- xvii. an “A” at PHM13773-6,
- xviii. a “C” at PHM13773-11,
- xix. an “A” at PHM16422-11,
- xx. a “T” at PHM1147-19,
- xxi. a “G” at PHM5280-41,
- xxii. a “T” at PHM9301-37, and
- xxiii. a “T” at PHM4423-4;
- and
- c. selecting said maize plant that has the at least one marker allele.
5-13. (canceled)
A method of identifying a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising detecting in a maize plant a marker locus wherein:
- a. a marker probe comprising all or a portion of the marker locus, or complement thereof, hybridizes under stringent conditions to the contiguous DNA between and including SEQ ID NO:48, or a nucleotide sequence that is 95% identical to SEQ ID NO:48 based on the Clustal V method of alignment, and SEQ ID NO:55, or a nucleotide sequence that is 95% identical to SEQ ID NO:55 based on the Clustal V method of alignment;
- and
- b. said marker locus comprises at least one allele that is associated with the enhanced resistance to Fusarium ear mold.
A method of identifying a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising detecting in the germplasm of the maize plant at least one allele of a marker locus wherein:
- a. the marker locus is located within a chromosomal interval comprising and flanked by PHM4423 and PHM16422;
- and
- b. the marker locus comprises at least one allele that is associated with enhanced resistance to Fusarium ear mold.
A method of identifying a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising detecting in the germplasm of the maize plant a haplotype comprising alleles at one or more marker loci, wherein:
- a. the one or more marker loci are located within a chromosomal interval comprising and flanked by PHM4423 and PHM16422;
- and
- b. the haplotype is associated with enhanced resistance to Fusarium ear mold.
A method of selecting a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising:
- a. obtaining a first maize plant having at least one allele of a marker locus, wherein the marker locus is located within a chromosomal interval comprising and flanked by PHM4423 and PHM16422 and the allele is associated with enhanced resistance to Fusarium ear mold;
- b. crossing said first maize plant to a second maize plant;
- c. evaluating progeny plants for the allele of the first maize plant;
- and
- d. selecting progeny plants that possess the allele of the first maize plant.
A method of selecting a maize plant that displays enhanced resistance to Fusarium ear mold, the method comprising:
- a. obtaining a first maize plant that comprises within its genome:
- i. a “T” at PHM4423-4, a “T” at PHM11850-3, and an “A” at PHM13773-6;
- or
- ii. an “A” at PHM9362-8 and an “A” at PHM13773-6;
- b. crossing said first maize plant to a second maize plant;
- c. evaluating progeny plants for said alleles;
- and
- d. selecting progeny plants that possess said alleles.
(canceled)
(canceled)
Dependencies are inferred from the claim text.