Methods and Systems for Visualizing and Evaluating Data
AssigneeLife Technologies Corporation
InventorsGordon A. Janaway, Manjula Aliminati, Ruoyun Wu, David Fortescue, Ming Shen
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Abstract
A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result is provided. The method includes detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period. The method further includes determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data. A dPCR result is generated based on the positive or negative amplification determinations for the plurality of samples.
Claims29
A computer-implemented method of generating a digital polymerase chain reaction (dPCR) result, the method comprising:
- detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period;
- determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the first set of emission data;
- and
- generating a dPCR result based on the positive or negative amplification determinations for the plurality of samples.
A computer-readable medium encoded with instructions, executable by a processor, the instructions comprising instructions for:
- detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period;
- detecting a second set of emission data from the plurality of samples at a second time during an amplification period, wherein the first and second set of emission data is included in amplification curve data for each of the plurality of samples;
- determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the corresponding amplification curve data;
- and
- generating a dPCR result based on the positive or negative amplification determinations for the plurality of samples.
A system for generating a digital polymerase chain reaction result (dPCR), the system comprising:
- a thermal cycler for performing amplification on a plurality of samples;
- a processor;
- and
- a memory encoded with instructions for:
- detecting a first set of emission data from a plurality of samples, each included in a sample region of a plurality of sample regions, at a first time during an amplification period;
- detecting a second set of emission data from the plurality of samples at a second time during an amplification period, wherein the first and second set of emission data is included in amplification curve data for each of the plurality of samples;
- determining a positive or negative amplification determination for each sample of the plurality of samples based in part on the corresponding amplification curve data;
- and
- generating a dPCR result based on the positive or negative amplification determinations for the plurality of samples.
Dependencies are inferred from the claim text.