Linear expression cassettes and uses thereof
OwnerKate Broderick, Feng Lin +2inventors
InventorsKate Broderick, Feng Lin, Niranjan Sardesai, Xuefei Shen
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Abstract
Provided herein are linear nucleic expression cassettes and methods of using same in a non-invasive method of vaccination. The method combines electroporation and linear DNA constructs encoding antigens to elicit antigen-specific immune responses.
Claims12
A nucleic acid construct capable of expressing a desired antigen in cells of a subject, comprising:
- a linear expression cassette comprising an antigen encoding sequence selected from the group consisting of SEQ ID NOs:
- 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 38, 44, 46, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, and sequences that are at least 98% similar thereof;
- or antigen encoding sequence encoding an antigen selected from the group consisting of SEQ ID NOs:
- 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 39-43, 45, 47, 48-193, 195, 197, 199, 201, 203, 205, 207, 209, 211, 213, 215, 217, 219, 221, 223, 225, 227, 229, 231, 233, 235, 237, 239, 241, 243, 245, 247, and 249, and sequences that are at least 98% similar thereof.
A non-invasive method of vaccination comprising electroporating a linear expression cassette into a subject in need thereof, wherein the linear expression cassette comprises a nucleic acid encoding one or more antigens.
A vaccination kit comprising an electroporation device and a linear expression cassette, wherein the linear expression cassette comprises a nucleic acid encoding one or more antigens.
Dependencies are inferred from the claim text.