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US 2014/0235846 A1Published application
Polyelectrolyte-Coated Ion-Exchange Particles
AssigneeApplied Biosystems, LLC
InventorsMichael Harrold, Aldrich Lau
Life of this patent
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Abstract
A polyelectrolyte-coated particle, devices for using the particle, methods for using the particle for separating PCR reaction products and/or DNA sequencing reaction products, and compositions for coating the particle are provided.
Claims27
A particle for separating PCR reaction products comprising:
- a core comprising ion-exchange material;
- and
- a coating covering the exterior surface of the particle wherein the coating comprises a linear polyelectrolyte polymer, wherein the polyelectrolyte polymer creates a size exclusion barrier allowing small molecules having a size less than 300 bp to penetrate into the particle and restricting large molecules from interacting with the core;
- and wherein the particle is produced by exposing the core to an excess of the polyelectrolyte polymer.
1 more element
-24. (canceled)
A particle for separating DNA sequencing reaction products, comprising:
- a core comprising ion-exchange material;
- and
- a coating covering the entire exterior surface of the particle wherein the coating comprises a linear polyelectrolyte polymer, wherein the polyelectrolyte polymer creates a size exclusion barrier allowing small molecules to penetrate into the particle and substantially excluding dye-labelled ssDNA fragments having greater than 45 nucleotides from interacting with the core;
- and wherein the particle is produced by exposing the core to an excess of the polyelectrolyte polymer.
1 more element
-29. (canceled)
-44. (canceled)
A method of purifying DNA sequencing reaction products, the method comprising:
- providing a plurality of particles, wherein each particle comprises a core for ion-exchange and a coating of polyelectrolyte;
- and
- contacting the DNA sequencing reaction products to separate dye-labeled ssDNA fragments.
-65. (canceled)
Dependencies are inferred from the claim text.