Process for producing novel sialo-sugar chain
AssigneeWakayama University
InventorMasanori Yamaguchi
Life of this patent
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Abstract
The present invention provides a novel sialo-sugar chain, a process for producing the sialo-sugar chain, and a device for producing the sialo-sugar chain. A sialo-sugar chain can be easily and efficiently mass-produced by reacting a sugar wherein a hydroxy groups is substituted with an alkynyl group (herein sometimes referred to as “alkynylated sugar”) with a specific sialic acid donor in the presence of a sialic acid-introducing enzyme.
Claims11
A compound represented by formula (5):
- wherein X is a sugar residue obtained by removing hydroxy from hydroxy group at the reducing end of a monosaccharide or an oligosaccharide, and removing hydrogen from hydroxy at a non-reducing end thereof;
- R9 is hydrogen or a group that acylates an amino group;
- R10 is the same or different, and each represents hydrogen or a hydroxy-protecting group;
- and n is an integer of 1 to 10.
A compound represented by formula (7a):
- wherein X represents a sugar residue obtained by removing hydroxy from hydroxy at the reducing end of a monosaccharide or an oligosaccharide, and removing hydrogen from hydroxy at a non-reducing end thereof;
- R9 is hydrogen or a group that acylates an amino group;
- R10 is the same or different, and each represents hydrogen or a hydroxy-protecting group;
- n is an integer of 1 to 10;
- and R1 is an organic group,
- or represented by formula (7b):
- wherein X represents a sugar residue obtained by removing hydroxy from hydroxy at the reducing end of a monosaccharide or an oligosaccharide, and removing hydrogen from hydroxy at a non-reducing end thereof;
- R9 is hydrogen or a group that acylates an amino group;
- R10 is the same or different, and each represents hydrogen or a hydroxy-protecting group;
- n is an integer of 1 to 10;
- and R1 is an organic group.
A process for producing a compound represented by formula (5):
- wherein X represents a sugar residue obtained by removing hydroxy from hydroxy at the reducing end of a monosaccharide or an oligosaccharide, and removing hydrogen from hydroxy at a non-reducing end thereof;
- R9 is hydrogen or a group that acylates an amino group;
- R10 is the same or different, and each represents hydrogen or a hydroxy-protecting group;
- and n is an integer of 1 to 10,
- the process comprising reacting a sialic acid donor and a compound represented by formula (3):
- wherein X represents a sugar residue obtained by removing hydroxy from hydroxy at the reducing end of a monosaccharide or an oligosaccharide, and removing hydrogen from hydroxy at a non-reducing end thereof;
- and n is an integer of 1 to 10,
- in the presence of a sialic acid-introducing enzyme.
A device comprising a column (hereinafter referred to as “column C”) having an inlet and an outlet, the column containing sialidase immobilized on a carrier.
Dependencies are inferred from the claim text.