Method for purifying protein
AssigneeAsahi Kasei Chemicals Corporation
InventorsYuta Sato, Naoyuki Shinohara, Hironobu Shirataki
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Abstract
It is an object of the present invention to provide a method for eluting an adsorbed protein that suppresses a decrease in protein adsorption ability, in a method for purifying a protein using a protein-adsorbing porous membrane. The present invention provides a method for purifying a protein, comprising an adsorption step and an elution step, wherein in the elution step, at least one eluent is passed in the opposite direction with respect to the direction of the passage of a stock solution containing an adsorption target protein, in the adsorption step.
Claims14
A method for purifying a protein by a porous membrane having a substrate surface coated with a polymer having protein adsorption ability, comprising:
- an adsorption step of passing a stock solution containing an adsorption target protein through the porous membrane to allow the polymer to adsorb the adsorption target protein;
- and
- an elution step of passing an eluent through the porous membrane to allow the adsorption target protein that is adsorbed on the polymer to elute in the eluent,
- wherein in the elution step, at least one eluent is passed in an opposite direction with respect to a direction of passage of the stock solution in the adsorption step.
Dependencies are inferred from the claim text.