LENTIVIRAL VECTORS PSEUDOTYPED WITH MUTANT BaEV GLYCOPROTEINS
AssigneesEcole Normale Superieure De Lyon, Institut National Dela Sante Et De La Recherche Medicale (Inserm)
InventorsAnais Girard-Gagnepain, Els Verhoeyen, Dimitri Lavillette, François-Loïc Cosset
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Abstract
The present invention concerns a pseudotyped viral vector particle for transferring biological material into cells, wherein said vector particle comprises at least:—a chimeric envelope glycoprotein which comprises or consists in a fusion of the transmembrane and extracellular domain of a baboon endogenous retrovirus (BaEV) envelope glycoprotein and the cytoplasmic tail domain of a murine leukemia virus (MLV) envelope glycoprotein; or—a modified BaEV envelope glycoprotein wherein the cytoplasmic tail domain is devoid of the fusion inhibitory R peptide.
Claims15
A pseudotyped viral vector particle for transferring biological material into cells, wherein said vector particle comprises at least:
- a chimeric envelope glycoprotein which comprises or consists in a fusion of the transmembrane and extracellular domain of a baboon endogenous retrovirus (BaEV) envelope glycoprotein and the cytoplasmic tail domain of a murine leukemia virus (MLV) envelope glycoprotein;
- or
- a modified BaEV envelope glycoprotein wherein the cytoplasmic tail domain is devoid of the fusion inhibitory R peptide.
A method for producing a pseudotyped viral vector particle comprising:
- a) transfecting a cell with:
- (i) at least one first nucleic acid sequence comprising a packaging competent retroviral derived genome;
- (ii) at least one second nucleic acid sequence comprising a cDNA encoding core proteins from said retrovirus, and
- (iii) at least one third nucleic acid sequence comprising a cDNA encoding:
- a chimeric envelope glycoprotein which comprises or consists in a fusion of the transmembrane and extracellular domain of a baboon endogenous retrovirus (BaEV) envelope glycoprotein and the cytoplasmic tail domain of a murine leukemia virus (MLV) envelope glycoprotein, as defined above;
- or
- a modified BaEV envelope glycoprotein wherein the cytoplasmic tail domain is devoid of the fusion inhibitory R peptide, as defined above;
- to yield a producer cell;
- b) maintaining the producer cell in culture for sufficient time to allow expression of the cDNAs to produce the encoded proteins;
- and
- c) allowing the encoded proteins to form pseudotyped viral vector particles.
(canceled)
Dependencies are inferred from the claim text.