Antisense modulation of ptp1b expression
AssigneeIsis Pharmaceuticals, Inc.
InventorsSanjay Bhanot, Susan M. Freier
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Abstract
Provided herein are methods, compounds, and compositions for reducing expression of PTP1B mRNA and protein in an animal. Such methods, compounds, and compositions are useful to treat, prevent, delay, or ameliorate metabolic disease, for example, diabetes, or a symptom thereof.
Claims33
1-56. (canceled)
A method of treating diabetes in a subject comprising administering to the subject a pharmaceutical composition suitable for parenteral administration to an animal comprising an aqueous solution, said aqueous solution comprising:
- a pharmaceutically acceptable carrier or diluent;
- and
- a single stranded modified oligonucleotide targeted to PTP1B consisting of 20 linked nucleosides having a nucleobase sequence consisting of SEQ ID NO:
- 26, or salt thereof,
- wherein the carrier or diluent is sterile and the aqueous solution is suitably viscous for parenteral administration and wherein the composition is administered parenterally, thereby treating diabetes in the subject.
A method of decreasing blood glucose levels in a subject comprising administering to the subject a compound comprising a single stranded modified oligonucleotide targeted to PTP1B consisting of 20 linked nucleosides having a nucleobase sequence consisting of SEQ ID NO:
- 26, wherein the modified oligonucleotide comprises:
- a gap segment consisting of ten linked deoxynucleosides;
- a 5′ wing segment consisting of five linked nucleosides;
- and
- a 3′ wing segment consisting of five linked nucleosides;
- wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment;
- each nucleoside of each wing segment comprises a 2′-O-methoxyethyl sugar;
- each internucleoside linkage is a phosphorothioate linkage;
- and each cytosine of the modified oligonucleotide is a 5-methylcytosine;
- and
- wherein the compound is administered to the subject parenterally, thereby decreasing blood glucose levels in the subject.
Dependencies are inferred from the claim text.