Means and methods for rhamnolipid production
AssigneesTechnische Universitaet Dortmund, Heinrich-Heine-Universitaet
InventorsLars Blank, Frank Rosenau, Susanne Wilhelm, Andreas Wittgens, Till Tiso
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Abstract
Provided is a host cell comprising a rhlA gene or an ortholog thereof, under the control of a heterologous promoter and a rhlB gene or an ortholog thereof, under the control of a heterologous promoter. The host cell is capable of achieving a carbon yield of more than 0.18 Cmol rhamnolipid/Cmol substrate. Provided is also a method of producing rhamnolipids, employing such a host cell.
Claims33
A host cell comprising
- (a) a rhlA gene or an ortholog thereof, being under the control of a heterologous promoter;
- and
- (b) a rhlB gene or an ortholog thereof, being under the control of a heterologous promoter,
- wherein said host cell is capable of achieving a carbon yield of more than 0.18 Cmol rhamnolipid/Cmol substrate.
A method of producing a rhamnolipid, the method comprising culturing a host cell at a temperature above 30° C. in a suitable medium and allowing the host cell to produce the rhamnolipid, wherein the host cell comprises a rhlA gene, or an ortholog thereof, and a rhlB gene, or an ortholog thereof.
(canceled)
Dependencies are inferred from the claim text.