Nucleic acid linker
AssigneesNational University Corporation Saitama University, Nikon Corporation
InventorsNaoto Nemoto, Shingo Ueno, Hirofumi Shiono
Life of this patent
Estimating the term…
Abstract
A nucleic acid linker is for producing a complex of mRNA and a protein encoded by that mRNA, comprising one 3′-terminal region and two branched 5′-terminal regions, wherein the 3′-terminal region comprises a single-stranded polynucleotide segment able to hybridize with the sequence on the 3′-terminal side of the mRNA, and an arm segment that branches off from the single-stranded polynucleotide segment and has a linking segment with the protein on the terminal thereof, and one of the two 5′-terminal regions has a binding site with the 3′-terminal of the mRNA.
Claims15
A nucleic acid linker for producing a complex of mRNA and a protein encoded by that mRNA, comprising:
- one 3′-terminal region, and
- two branched 5′-terminal regions;
- wherein,
- the 3′-terminal region comprises a single-stranded polynucleotide segment able to hybridize with the sequence on the 3′-terminal side of the mRNA, and
- an arm segment that branches off from the single-stranded polynucleotide segment and has a linking segment with the protein on the terminal thereof, and
- one of the two 5′-terminal regions has a binding site with the 3′-terminal of the mRNA.
A nucleic acid linker for producing a complex of mRNA and a protein encoded by that mRNA, provided with:
- a 3′-terminal region containing a single-stranded polynucleotide segment able to hybridize with the sequence on the 3′-terminal side of the mRNA,
- two branched 5′-terminal regions, and
- an arm segment having a linking segment with the protein on the terminal thereof;
- wherein,
- at least one of the two 5′-terminal regions has a spacer region containing a solid phase binding site on the 5′-terminal thereof.
Dependencies are inferred from the claim text.