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US 2014/0235506 A1Published application
Polynucleotide barcode generation
Assignee10X Technologies, Inc.
InventorsBenjamin Hindson, Mirna Jarosz, Paul Hardenbol, Michael Schnall-Levin, Kevin Ness, Serge Saxonov
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.
Claims31
-62. (canceled)
A library, comprising:
- a plurality of nucleic acid barcode sequences comprising at least 1000 different nucleic acid barcode sequences;
- a plurality of partitions comprising at least 1000 partitions, wherein each of at least 1000 different nucleic acid barcode sequences in the plurality of barcode sequences is disposed within a separate partition;
- and
- wherein each of at least 1000 partitions of the plurality of partitions comprises more than 1000 nucleic acid barcode molecules comprising the same nucleic acid barcode sequence.
1 more element
Dependencies are inferred from the claim text.