Method of applying etfb to abnormal proliferation of cell, and abnormal proliferation inhibitor
AssigneePola Pharma Inc.
InventorsShigenari Hirokawa, Hiroyuki Kitajima, Tomomasa Shimanuki
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Abstract
Provided is a method for identifying and suppressing abnormal growth of fibroblasts at an early stage. Provided is a method for identifying the growth state of fibroblasts using as an index the level of expression of ETFB (electron transfer flavoprotein beta subunit), comprising: judging, in cases where the level of expression of ETFB is high, that there is a high probability that fibroblasts are abnormally growing; and judging, in cases where the level of expression of ETFB is low, that there is a high probability that fibroblasts are normally growing. Further, by inhibition of ETFB, abnormal growth of fibroblasts can be suppressed.
Claims22
A method for identifying the growth state of fibroblasts using as an index the level of expression of ETFB (electron transfer flavoprotein beta subunit) in collected fibroblasts, comprising:
- judging, in cases where the level of expression of ETFB 15 high, that there is a high probability that fibroblasts are abnormally growing, and
- judging, in cases where the level of expression of ETFB 15 low, that there is a high probability that fibroblasts are normally growing.
A method for identifying fibrosis in an organ suspected of having fibrosis, using as an index the level of expression of ETFB in fibroblasts collected from the organ, comprising:
- judging, in cases where the level of expression of ETFB 15 high, that there is a high probability that the organ has fibrosis;
- and
- judging, in cases where no rise in ETFB 15 found, that there is only a low probability that the organ has fibrosis.
A method for identifying a fibrosis-suppressing agent, comprising:
- culturing fibroblasts under tension in a collagen gel in the presence and absence of a test substance;
- and
- judging the test substance to be a fibrosis-suppressing agent in cases where the expression level of ETFB is lower in the culture in the presence of the test substance than in the culture in the absence of the test substance.
A fibrosis-suppressing method comprising:
- administering to a subject in need thereof a test substance, wherein, when fibroblasts are cultured under tension in a collagen gel in the presence and absence of the test substance, the expression level of ETFB is lower in the culture in the presence of the test substance than in the culture in the absence of the test substance.
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Dependencies are inferred from the claim text.