Methods And Devices For Amplification Of Nucleic Acid
AssigneeIntelligent BioSystems, Inc.
InventorsSteven Gordon, Daniel Stetson
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Abstract
The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.
Claims31
A method of amplifying and sequencing nucleic acid, comprising:
- a) providing i) a population of different nucleic acid template molecules, ii) a plurality of first and second single stranded oligonucleotides immobilized on a glass surface, iii) amplification reagents, iv) sequencing reagents, and v) a plurality of first and second sequencing primers;
- b) hybridizing at least a portion of said population of nucleic acid template molecules to said plurality of first oligonucleotides immobilized on said glass surface;
- c) amplifying said nucleic acid template molecules so as to create a plurality of forward and reverse single stranded strands;
- d) sequencing said forward oligonucleotide strands with said first sequencing primers;
- and
- e) sequencing said reverse oligonucleotide strands with said second sequencing primers.
A method of amplifying and sequencing nucleic acid, comprising:
- a) providing i) a population of different nucleic acid template molecules, ii) a plurality of first and second single stranded oligonucleotides immobilized on a glass surface, iii) amplification reagents, iv) sequencing reagents, and v) a plurality of first and second sequencing primers;
- b) hybridizing at least a portion of said population of nucleic acid template molecules to said plurality of first oligonucleotides immobilized on said glass surface;
- c) amplifying said nucleic acid template molecules so as to create a plurality of forward strands;
- d) sequencing said forward oligonucleotide strands with said first sequencing primers;
- e) amplifying said nucleic acid template molecules so as to create a plurality of reverse single stranded strands;
- and
- f) sequencing said reverse oligonucleotide strands with said second sequencing primers.
A method of sequencing nucleic acid, comprising:
- a) providing i) a plurality of different amplicons immobilized on a first surface enclosed in a chamber, and ii) sequencing reagents;
- b) introducing said sequencing reagents into said chamber;
- and
- c) performing sequencing by synthesis.
A method of sequencing nucleic acid, comprising:
- a) providing i) a plurality of different amplicons immobilized on a plurality of beads, said beads enclosed in a chamber, said chamber sealed by an adhesive gasket sandwiched between a first surface and a second surface and ii) sequencing reagents;
- b) introducing said sequencing reagents into said chamber;
- and
- c) performing sequencing by synthesis.
A device, comprising a plurality of different nucleic acid amplicons immobilized in regions on a glass surface, wherein the amplicon within each region is homogeneous.
Dependencies are inferred from the claim text.