Measurement and Monitoring of Cell Clonality
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsAndrew Fire, Scott D. Boyd, Jason Merker, Kyunga Seo, Eleanor M. Osborne
Life of this patent
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Abstract
Methods are provided for the detection and analysis of clonality in a cell population, where parallel sequencing is applied to a nucleic acid sample obtained from the cell population, optionally a population of lymphocytes. Replicate samples are amplified, and sequenced, where identification of coincident sequences in two or more replicates is indicative of clonal expansion.
Claims8
1-105. (canceled)
A method of determining the responsiveness of an individual to a vaccine, the method comprising:
- obtaining a cell sample from said individual in a short defined time period following immunization with said vaccine;
- determining sequences from a locus of interest in at least two distinct pools of nucleic acid from the sample;
- comparing said sequences to detect the presence of coincident sequences in at least two distinct pools of nucleic acid;
- wherein the presence of coincident sequences is indicative of clonal expansion and responsiveness to said vaccine.
(canceled)
110-111. (canceled)
113-114. (canceled)
Dependencies are inferred from the claim text.