Th17 differentiation markers for acne and uses thereof
OwnerIsabelle Carlavaninventor
Life of this patent
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Abstract
A method is described for using ROR gamma t or ROR alpha to diagnose acne and/or to screen inhibitors of Th17 differentiation. Specifically described are methods of inhibiting ROR gamma t or ROR alpha and use of the screened inhibitors in acne treatment.
Claims12
An acne marker, the marker comprising DNA or mRNA encoding ROR gamma t, or a corresponding protein.
An acne marker, the marker comprising DNA or mRNA encoding ROR alpha, or a corresponding protein.
An acne marker, the marker comprising DNA or mRNA encoding ROR gamma t or a corresponding protein, DNA or mRNA encoding ROR alpha or a corresponding protein and/or at least one marker selected from the group consisting of IL-6, IL-17A, IL-17F, IL21, IL-22, IL-23A, IL-26, and CCL20.
A method for monitoring progression or variation of acne, the method comprising the following steps of:
- a) taking a biological sample from an individual,
- b) analyzing a level of expression of at least one proposed markers, and/or at least one marker selected from the group consisting of IL-6, IL-17A, IL-17F, IL-22, and CCL20 in a sample and in which a variation in expression of at least one of the markers is an indicator of the progression of acne.
An in vitro screening method of Th17 cells differentiation inhibitors for identifying drug candidates, the method comprising the following steps of:
- a) collecting at least two biological samples:
- wherein one sample mimics an acne lesion, and the other sample mimics a healthy condition;
- b) contacting at least one sample or a mixture of samples with one or more drug candidates to be tested;
- c) detecting expression or biological function of at least one proposed markers, and/or at least one expression marker selected from the group consisting of:
- IL-17A, IL-17F, IL-22, and CCL20 in the biological sample or mixture obtained in b);
- d) selecting drug candidates that inhibit expression or biological function of IL-17A, IL-17F, IL-22, CCL20 measured in said sample or mixture obtained in b) and comparing the levels with a sample not mixed with the drug candidate.
Dependencies are inferred from the claim text.