Methods and compositions for the detection of balanced reciprocal translocations/rearrangements
AssigneeUniversity of Utah Research Foundation
InventorsShashirekha Shetty, Rong Mao
Life of this patent
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Abstract
Disclosed are methods and compositions related to the detection of balanced reciprocal translocations/rearrangements and determination of the location of said balanced reciprocal translocations/rearrangements. Also disclosed are methods and compositions relating to the diagnosis of a disease or condition associated with uncontrolled cellular proliferation by the detection of balanced reciprocal translocation/rearrangement. Additionally, also disclosed are kits for the detection of balanced reciprocal translocations/rearrangements.
Claims25
A method of detecting the presence of a chromosomal rearrangement in a test sample comprising:
- (a) isolating a first genomic DNA from cells of a test sample and a second genomic DNA from cells of a reference sample;
- (b) hybridizing the test and reference DNA products to a tiling density DNA microarray comprising genomic DNA sequences, wherein the there is a 3-12 base pair overlap of DNA oligomeric probes;
- and
- (c) comparing the pattern and extent of hybridization of the test DNA product with the reference DNA product to the DNA microarray;
- wherein a patterned loss and recovery of hybridization of the test sample DNA product over the reference sample DNA product to a DNA microarray element indicates the presence of chromosomal rearrangement.
A method of diagnosing of a disease or condition associated with chromosomal rearrangement in a test sample comprising:
- (a) isolating a first genomic DNA from cells of a test sample and a second genomic DNA from cells of a reference sample;
- (b) hybridizing the test and reference DNA products to a tiling density DNA microarray comprising genomic DNA sequences, wherein the there is a 3-12 base pair overlap of DNA oligomeric probes;
- and
- (c) comparing the pattern and extent of hybridization of the test DNA product with the reference DNA product to the DNA microarray;
- wherein a patterned loss and recovery of hybridization of the test sample DNA product over the reference sample DNA product to a DNA microarray element indicates the presence of chromosomal rearrangement;
- and wherein the presence of a chromosomal rearrangement at a location associated with a disease or condition indicates the presence of the disease or condition.
A kit for use in the detection of a balanced chromosomal translocation/rearrangement, the kit comprising:
- (a) an array for the detection of product and (b) the reagents needed for dye labeling and CGH microarray hybridization.
An array for CGH analysis of a chromosomal rearrangement associated with a disease.
Dependencies are inferred from the claim text.