Systems and methods for genetic and biological analysis
AssigneeGenapsys, Inc.
InventorsHesaam Esfandyarpour, Kosar Baghbani Parazi, Mark F. Oldham, Eric S. Nordman, Richard T. Reel, Susanne Baumhueter, Cheryl Heiner, Frank Lee
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Abstract
The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
Claims34
-36. (canceled)
A method for sequencing a nucleic acid sample, comprising:
- a. providing a plurality of particles adjacent to a sensor array, wherein an individual particle of said plurality is positioned adjacent to an individual sensor of said sensor array and is coupled to a nucleic acid molecule generated from said nucleic acid sample, and wherein said individual sensor provides a virtual wall during sequencing that isolates said individual sensor from other sensors of said sensor array;
- b. hybridizing a primer to said nucleic acid molecule;
- c. performing a primer extension reaction by contacting said nucleic acid molecule with nucleotide bases in the presence of a polymerase adjacent to said nucleic acid molecule;
- d. detecting signals indicative of incorporation events associated with one or more of said nucleotide bases during said primer extension reaction;
- e. monitoring and correcting for phase error introduced during said primer extension reaction;
- and
- f. generating a sequence of said nucleic acid sample at reduced phase error.
A method for sequencing a nucleic acid sample at reduced phase error, comprising:
- a. providing a plurality of particles adjacent to a sensor array, wherein an individual particle of said plurality is positioned adjacent to an individual sensor of said sensor array and is coupled to a nucleic acid molecule generated from said nucleic acid sample;
- b. hybridizing a primer to said nucleic acid molecule;
- c. performing a primer extension reaction by contacting said nucleic acid molecule with nucleotide bases in the presence of a polymerase adjacent to said nucleic acid molecule;
- d. using said individual sensor, measuring local impedance changes within a Debye layer associated with said individual particle during said primer extension reaction;
- e. monitoring and correcting for phase error introduced during said primer extension reaction;
- and
- f. generating a sequence of said nucleic acid sample at reduced phase error.
A sensing system, comprising:
- an array of sensors, wherein an individual sensor of said array comprises at least two electrodes that, during sensing using said individual sensor, are disposed adjacent to a particle comprising a nucleic acid molecule coupled thereto, wherein during sensing said at least two electrodes are coupled to a Debye layer associated with said particle,
- wherein said individual sensor is configured to generate a virtual wall to control the movement of said particle or said nucleic acid molecule(s) during sensing, and
- wherein said individual sensor is configured to control a shape or period of said virtual wall.
A method for sequencing a nucleic acid sample, comprising:
- a. providing a plurality of particles adjacent to a sensor array, wherein an individual particle of said plurality is positioned adjacent to an individual sensor of said sensor array and is coupled to a nucleic acid molecule generated from said nucleic acid sample;
- b. hybridizing a primer to said nucleic acid molecule;
- c. performing a primer extension reaction by contacting said nucleic acid molecule with nucleotide bases in the presence of a polymerase adjacent to said nucleic acid molecule;
- and
- d. sequencing said nucleic acid sample by detecting signals indicative of incorporation events associated with one or more of said nucleotide bases during said primer extension reaction, which sequencing employs steady state electronic sequencing.
Dependencies are inferred from the claim text.