Tagged ligands for enrichment of rare analytes from a mixed sample
AssigneeGPB Scientific, LLC
InventorsAllyn FORSYTH, Helen BARNES
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Abstract
Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.
Claims12
A method for enriching rare analytes from a sample comprising:
- contacting in solution said sample with a plurality of affinity-tagged ligands (ATLs) wherein said mixture of ATL's comprises:
- a first ATL comprising a first ligand that selectively binds a first marker of said rare analytes, wherein said first ligand is linked to a first affinity tag that is selectively captured by a first capture moiety;
- and
- a second ATL comprising a second ligand that selectively binds a second marker of said rare analytes, wherein said second ligand is linked to a second affinity tag, wherein said second affinity tag is selectively captured by said first capture moiety;
- and
- contacting said sample with said capture moiety to selectively enrich said rare analytes.
Dependencies are inferred from the claim text.