Rapid Protein Labeling and Analysis
AssigneeLife Technologies Corporation
InventorsThomas DILLER, Bethany Sutton, George Hanson, Timothy Updyke
Life of this patent
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Abstract
The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.
Claims7
1-34. (canceled)
A kit for fluorescently labeling a mixture of proteins comprising a tagged protein, said kit comprising:
- a) a first composition comprising a first buffer having a pH in the range from pH 8 to pH 10, an amine reactive fluorogenic reagent or an amine reactive fluorescent dye, a sugar or a sugar alcohol, and an anionic surfactant, wherein said first buffer does not include buffer components or additives that have primary or secondary amine moieties;
- and
- b) a second composition comprising a second buffer in the range from pH 6 to pH 10, and optionally a tag binding fluorogenic reagent.
Dependencies are inferred from the claim text.