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US 2014/0234975 A1Published application
Method for increasing the efficiency of double-strand-break induced mutagenesis
AssigneeCellectis
InventorsGeorge H Silva, Rachel Macmaster
Life of this patent
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Abstract
The present invention relates to a method for increasing double-strand-break induced mutagenesis at a genomic locus of interest in a cell, thereby giving new tools for genome engineering, including therapeutic applications and cell line engineering. More specifically, the present invention concerns the combined use of TALEN or meganucleases with TREX2, especially under the form of single-chain proteins.
Claims17
1-13. (canceled)
A polynucleotide encoding a polypeptide that comprises:
- (i) a first polypeptide that is a human TREX2 protomer (SEQ ID NO:
- 26) or functional mutant thereof that shares at least 80% identity with the human TREX2 exonuclease protomer;
- (ii) a second polypeptide that is a human TREX2 protomer (SEQ ID NO:
- 26) or functional mutant thereof that shares at least 80% identity with the human TREX2 exonuclease protomer;
- and
- (iii) a peptidic linker connecting said first and second polypeptides.
3 more elements
A polynucleotide encoding a polypeptide that comprises:
- (i) a first polypeptide that is a human TREX2 protomer (SEQ ID NO:
- 26) or functional mutant thereof that shares at least 80% identity with the human TREX2 exonuclease protomer;
- (ii) a second polypeptide that is a human TREX2 protomer (SEQ ID NO:
- 26) or functional mutant thereof that shares at least 80% identity with the human TREX2 exonuclease protomer;
- (iii) a peptidic linker connecting said first and second polypeptides;
- and
- (iv) a third polypeptide sharing at least 80% identity with a rare cutting endonuclease.
4 more elements
Dependencies are inferred from the claim text.