Novel Methods and Formulations for Orthopedic Cell Therapy
AssigneeLineage Cell Therapeutics, Inc.
InventorsMichael West, Hal Sternberg
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Abstract
Aspects of the present invention include methods and compositions related to the production and use of clonal lineages of embryonic progenitor cell lines derived from differentiating cultures of primordial stem cells, in particular, said methods and compositions relate to methods of differentiating cells in the presence of BMP family mambeis of growth factors and the applications of said cell lines in the treatment of degenerative orthopedic diseases such as osteoarthritis,
Claims13
A method of increasing the expression of collagen II and minimizing the expression of collagen X in embryonic progenitor cell lines derived from pluripotent stem cells wherein the method comprises differentiating of said embryonic progenitor cells in the presence of GDF5 and TGFβ3 thereby increasing the expression of collagen II and minimizing the expression of collagen X compared to the embryonic progenitor cell not differentiated in the presence of GDF5 and TGFβ3.
A method of conditioning an embryonic progenitor cell line capable of chondrogenesis comprising culturing said embryonic progenitor cell line in the presence of one or more BMP factors, wherein said conditioning increases the expression of collagen II and minimizes the expression of collagen X in said embryonic progenitor cell lines compared to the embryonic progenitor cell line not cultured in the presence of one or more BMP factors.
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Dependencies are inferred from the claim text.