Substrates and methods for collection, stabilization and elution of biomolecules
AssigneeGeneral Electric Company
InventorsErnest William Kovacs, Erik Leeming Kvam, Bing Li, Frank John Mondello
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Abstract
A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.
Claims37
A solid substrate for extraction, stabilization and elution of biomolecules, comprising a melezitose under a substantially dry state.
A solid substrate for collection, stabilization and elution of biomolecules, comprising:
- a trisaccharide under a substantially dry state.
A method for extracting, stabilizing and eluting biomolecules from a biological sample disposed on a solid substrate, comprising:
- contacting the biological sample to the substrate, wherein the substrate comprises melezitose impregnated therein under a substantially dry state;
- drying the biological sample to a substantially dry state;
- and
- eluting the biomolecules from the biological sample dried on the substrate by rehydrating the substrate in an elution buffer.
Dependencies are inferred from the claim text.