Mutant rb69 dna polymerase
AssigneeLife Technologies Corporation
InventorsMarian PERIS, Michael PHELAN, Barnett ROSENBLUM, Stephen HENDRICKS
Life of this patent
Estimating the term…
Abstract
Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
Claims17
An isolated mutant DNA polymerase capable of selectively transiently-binding a labeled nucleotide and selectively incorporating a terminator nucleotide.
An isolated mutant DNA polymerase comprising an amino acid sequence that is at least 80%, 85%, 90%, 95%, 97% or 99% identical to any amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8.
Dependencies are inferred from the claim text.