Recombinant anaerobic acetogenic bacteria for production of isoprene and/or industrial bio-products using synthesis gas
AssigneesE. I. Du Pont de Nemours and Company, The Goodyear Tire & Rubber Company, Danisco US Inc.
InventorsZACHARY Q. BECK, Marguerite A. Cervin, Gopal K. Chotani, Bruce A. Diner, Janine Fan, Caroline M. Peres, Karl J. Sanford, Miles C. Scotcher, Derek H. Wells, Gregory M. Whited
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Abstract
This invention provides for recombinant anaerobic acetogenic bacterial cells having one or more nucleic acids whereby isoprene, mevalonate and/or other industrial bio-products are produced in a substantially oxygen-free culture condition using synthesis gas as energy and/or carbon source.
Claims28
Recombinant obligate anaerobic bacterial acetogen cells comprising:
- a) at least one heterologous nucleic acid molecule encoding an isoprene synthase polypeptide;
- b) at least one heterologous nucleic acid molecule encoding an isopentenyl diphosphate isomerase polypeptide,
- wherein the nucleic acid molecules of a) and b) are operably linked either separately or in an operon to a promoter that is functional in the cells, and
- wherein the cells produce more isoprene when grown in substantially oxygen-free culture conditions comprising synthesis gas as energy and/or carbon source, as compared to the cells without the heterologous nucleic acids.
Recombinant obligate anaerobic bacterial acetogen cells comprising at least one heterologous nucleic acid encoding at least two polypeptides having acetyl-CoA acetyltransferase 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, and 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase activities, wherein the heterologous nucleic acids are operably linked either separately or in an operon to a promoter that is active in the cells, and wherein the cells produce mevalonate when grown in substantially oxygen-free culture conditions comprising synthesis gas as energy and/or carbon source.
Recombinant obligate anaerobic bacterial acetogen cells capable of producing acetyl-CoA derived products, said cells comprising one or more heterologous nucleic acids encoding a polypeptide involved in the conversion of acetyl-CoA into a acetyl-CoA derived product in operable combination with a promoter, wherein the culturing of said cells under substantially oxygen-free culture conditions comprising synthesis gas as energy and/or carbon source provides for the production of said acetyl-CoA derived product.
Dependencies are inferred from the claim text.