Variants of glycerol dehydrogenase having d-lactate dehydrogenase activity and uses thereof
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsQingzhao Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Abstract
The present invention provides methods of designing and generating glycerol dehydrogenase (GlyDH) variants that have altered function as compared to a parent polypeptide. The present invention further provides nucleic acids encoding GlyDH polypeptide variants having altered function as compared to the parent polypeptide. Host cells comprising polynucleotides encoding GlyDH variants and methods of producing lactic acids are also provided in various aspects of the invention.
Claims30
1-28. (canceled)
An isolated polypeptide comprising the sequence of a glycerol dehydrogenase in which a phenylalanine has been substituted with an amino acid selected from serine, threonine, glycine or asparagine and/or an aspartic acid has been substituted with an amino acid selected from glutamine or asparagine, wherein said phenylalanine corresponds to the phenylalanine at position 245 of SEQ ID NO:
- 3 and said aspartic acid corresponds to position 121 of SEQ ID NO:
- 3.
An isolated nucleic acid comprising SEQ ID NO:
- 56 or tcgagaaaaa cttgacacat acCAAAAATA AGTTATGATG GAATTGTGCT TGTTATATTT TTCAC (nucleotides 84-148 of SEQ ID NO:
- 56).
An isolated nucleic acid comprising SEQ ID NO:
- 56 or tcgagaaaaa cttgacacat acCAAAAATA AGTTATGATG GAATTGTGCT TGTTATATTT TTCAC (nucleotides 84-148 of SEQ ID NO:
- 56) operably linked to a heterologous polynucleotide sequence.
Dependencies are inferred from the claim text.