Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same
AssigneeNovozymes A/S
InventorsSara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Abstract
The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims18
1-29. (canceled)
An isolated polypeptide having peroxygenase activity, selected from the group consisting of:
- (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2 comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has peroxygenase activity.
An enzymatic method for producing polyurethane, comprising converting an aliphatic hydrocarbon to a diol, according to the method of claim 55, and using the diol for producing polyurethane.
Dependencies are inferred from the claim text.