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US 2014/0234907 A1Published application
Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsYu Zhang, Ye Liu, Junxin Duan, Lan Tang
Life of this patent
Estimating the term…
Abstract
Provided are isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims15
1-17. (canceled)
An isolated polypeptide having endoglucanase activity, selected from the group consisting of:
- (a) a polypeptide having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, a polypeptide having at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 4;
- or a polypeptide having at least 88% sequence identity to the mature polypeptide of SEQ ID NO:
- 6;
- (b) a polypeptide encoded by a polynucleotide having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, a polypeptide encoded by a polynucleotide having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3, or a polypeptide encoded by a polynucleotide having at least 88% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5;
- (c) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, the mature polypeptide coding sequence of SEQ ID NO:
- 3, or the mature polypeptide coding sequence of SEQ ID NO:
- 5, (ii) the cDNA sequence of SEQ ID NO:
- 1, the cDNA sequence of SEQ ID NO:
- 3, or the cDNA sequence of SEQ ID NO:
- 5, or (iii) the full-length complement of (i) or (ii);
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2, a variant of the mature polypeptide of SEQ ID NO:
- 4, or a variant of the mature polypeptide of SEQ ID NO:
- 6, comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions, wherein the variant has endoglucanase activity;
- and
- (e) a fragment of a polypeptide of (a), (b), (c) or (d) that has endoglucanase activity.
19 more elements
An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
- (a) a catalytic domain having at least 70% sequence identity to amino acids 18 to 414 of SEQ ID NO:
- 2;
- a catalytic domain having at least 75% sequence identity to amino acids 26 to 400 of SEQ ID NO:
- 4;
- or a catalytic domain having at least 88% sequence identity to amino acids 21 to 422 of SEQ ID NO:
- 6;
- (b) a catalytic domain encoded by a polynucleotide having at least 70% sequence identity to nucleotides 52 to 1242 of SEQ ID NO:
- 1, a catalytic domain encoded by a polynucleotide having at least 75% sequence identity to nucleotides 76 to 1200 of SEQ ID NO:
- 3;
- or a catalytic domain encoded by a polynucleotide having at least 88% sequence identity to nucleotides 61 to 1358 of SEQ ID NO:
- 5;
- (c) a catalytic domain encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) nucleotides 52 to 1242 of SEQ ID NO:
- 1, nucleotides 76 to 1200 of SEQ ID NO:
- 3 or nucleotides 61 to 1358 of SEQ ID NO:
- 5, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (d) a variant of amino acids 18 to 414 of SEQ ID NO:
- 2, a variant of amino acids 26 to 400 of SEQ ID NO:
- 4, or a variant of amino acids 21 to 422 of SEQ ID NO:
- 6, comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions, wherein the variant has endoglucanase activity;
- and
- (e) a fragment of a catalytic domain of (a), (b), (c) or (d), which has endoglucanase activity.
18 more elements
An isolated polypeptide comprising a carbohydrate binding domain selected from the group consisting of:
- (a) a carbohydrate binding domain having at least 75% sequence identity to amino acids 427 to 463 of SEQ ID NO:
- 4;
- (b) a carbohydrate binding domain encoded by a polynucleotide having at least 75% sequence identity to nucleotides 1279 to 1389 of SEQ ID NO:
- 3;
- (c) a carbohydrate binding domain encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) nucleotides 1279 to 1389 of SEQ ID NO:
- 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (d) a variant of amino acids 427 to 463 of SEQ ID NO:
- 4, comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions, wherein the variant has carbohydrate binding activity;
- and
- (e) a fragment of the carbohydrate binding domain of (a), (b), (c) or (d), which has carbohydrate binding activity.
7 more elements
Dependencies are inferred from the claim text.