Alpha-Amylase Variants and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsCarsten Andersen, Signe Eskildsen Larsen, Esben Peter Friis, Peter Kamp Hansen, Henrik Friis Madsen, Anders Viksoe Nielsen, Pernille Ollendorf Micheelsen
Life of this patent
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Abstract
The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims19
An alpha-amylase variant comprising a substitution with proline at a position corresponding to position 185 of SEQ ID NO:
- 1 and further comprising a substitution at one or more positions corresponding to positions 15, 48, 49, 50, 107, 116, 133, 138, 156, 176, 181, 187, 188, 190, 197, 201, 205, 209, 213, 239, 241, 255, 264, 299, 360, 375, 416, 437, 474 and 475 of SEQ ID NO:
- 1, wherein the variant has at least 60% and less than 100% sequence identity to (i) the mature polypeptide of any of SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14, or (ii) amino acids 1 to 483 of SEQ ID NO:
- 1, amino acids 1 to 483 of SEQ ID NO:
- 2, amino acids 1 to 485 of SEQ ID NO:
- 3, amino acids 1 to 482 of SEQ ID NO:
- 4, amino acids 1 to 484 of SEQ ID NO:
- 5, amino acids 1 to 483 of SEQ ID NO:
- 6, amino acids 1 to 485 of SEQ ID NO:
- 7, amino acids 1 to 485 of SEQ ID NO:
- 8, amino acids 1 to 485 of SEQ ID NO:
- 9, amino acids 1 to 485 of SEQ ID NO:
- 10, amino acids 1 to 485 of SEQ ID NO:
- 11, amino acids 1 to 480 of SEQ ID NO:
- 12, amino acids 1 to 483 of SEQ ID NO:
- 13 or amino acids 1 to 481 of SEQ ID NO:
- 14, and wherein the variant has alpha-amylase activity.
(canceled)
A method for obtaining an alpha-amylase variant, comprising (a) introducing into a parent alpha-amylase a substitution with proline at a position corresponding to position 185 of SEQ ID NO:
- 1 and further a substitution at one or more positions corresponding to positions 15, 48, 49, 50, 107, 116, 133, 138, 156, 176, 181, 187, 188, 190, 197, 201, 205, 209, 213, 239, 241, 255, 264, 299, 360, 375, 416, 437, 474 and 475 of SEQ ID NO:
- 1;
- and (b) recovering the variant.
Dependencies are inferred from the claim text.