Method for harvesting photosynthetic unicells using genetically induced flotation
AssigneeUniversity of Wyoming
InventorsStephen K. Herbert, Levi G. Lowder
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Abstract
Methods for the harvesting of photosynthetic unicellular organisms are provided, including the formation and expression or overexpression of gas vesicles or vacuole proteins in photosynthetic unicellular organisms. DNA constructs as well as methods for integration of the DNA constructs into the genomes of photosynthetic unicellular organisms for the formation and expression or overexpression of gas vesicles or vacuole expression proteins in unicellular organisms are also disclosed.
Claims30
A DNA construct for the formation and expression or overexpression of gas vesicle protein coding sequences or vacuole protein coding sequences in photosynthetic unicells, wherein said DNA construct comprises a promoter and one operon, wherein said promoter is operably linked to said one operon, wherein said a single operon comprises gas vesicle expression protein coding sequences or vacuole expression protein coding sequences, wherein said gas vesicle expression protein coding sequences or vacuole expression protein coding sequences comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:15.
A DNA construct for the formation and expression or overexpression of gas vesicle protein coding sequences or vacuole protein coding sequences in photosynthetic unicells, wherein said DNA construct comprises a first promoter and first operon, and a second promoter and a second operon wherein said first promoter is operably linked to said first operon, and said second promoter is operably linked to said second operon wherein said first operon comprises gas vesicle expression protein coding sequences or vacuole expression protein coding sequences comprising SEQ ID NO:1, SEQ ID NO:3, and wherein said second operon comprises gas vesicle expression protein coding sequences or vacuole expression protein coding sequences comprising SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:15.
A DNA construct for the formation and expression or overexpression of gas vesicle protein coding sequences or vacuole protein coding sequences in photosynthetic unicellular organisms, wherein said DNA construct comprises the 5′ and 3′ UTRs of a gene of a chloroplast genome operably linked to the 5′ and 3′ end of heterologous operon coding sequence, wherein said gene of a chloroplast genome is a psbD gene and wherein said heterologous operon coding sequences comprises SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:11, SEQ ID NO:13 and SEQ ID NO:15.
Dependencies are inferred from the claim text.