Biosynthetic gene cluster for the production of peptide/protein analogues

A nucleic acid molecule or a composition of nucleic acid molecules comprising:

• (a) a nucleotide sequence of SEQ ID NO:
• 1;
• (b) a nucleotide sequence capable of hybridizing to the nucleotide sequence of SEQ ID NO:
• 1 under stringent hybridization conditions and encoding at least one polypeptide which catalyzes at least one step of the biosynthesis of polytheonamides and/or encoding a precursor peptide thereof;
• (c) a nucleotide sequence encoding at least one polypeptide or peptide selected from any one of poyA (SEQ ID NO:
• 3), poyB (SEQ ID NO:
• 5), poyC (SEQ ID NO:
• 7), poyD (SEQ ID NO:
• 9), poyE (SEQ ID NO:
• 11), poyF (SEQ ID NO:
• 13), poyG (SEQ ID NO:
• 15), poyH (SEQ ID NO:
• 17), poyI (SEQ ID NO:
• 19), poyK (SEQ ID NO:
• 21) and/or poyJ (SEQ ID NO:
• 23);
• (d) a nucleotide sequence encoding at least one polypeptide which catalyzes at least one step of the biosynthesis of polytheonamides and/or encoding a precursor peptide thereof which amino acid sequence is modified compared to the amino acid sequence of any one of poyA (SEQ ID NO:
• 3), poyB (SEQ ID NO:
• 5), poyC (SEQ ID NO:
• 7), poyD (SEQ ID NO:
• 9), poyE (SEQ ID NO:
• 11), poyF (SEQ ID NO:
• 13), poyG (SEQ ID NO:
• 15), poyH (SEQ ID NO:
• 17), poyI (SEQ ID NO:
• 19), poyK (SEQ ID NO:
• 21) and/or poyJ (SEQ ID NO:
• 23) by way of one or more amino acid substitution(s), deletion(s) and/or insertion(s);
• (e) a variant or portion of a nucleotide sequence of any one of (a) to (d) encoding at least one polypeptide which catalyzes at least one step of the biosynthesis of polytheonamides and/or encoding a precursor peptide thereof;
• (f) a nucleotide sequence which is degenerated with respected to the nucleotide sequence of any one of (a) to (e);
• or
• (g) a nucleotide sequence which is complementary to the nucleotide sequence in any one of (a) to (f).