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US 2014/0234899 A1Published application
Process for antibody g1 glycoform production
AssigneeHoffmann-La Roche Inc.
InventorsMartina Hueller, Dietmar Reusch
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Abstract
The current invention comprises a method for producing an immunoglobulin or immunoglobulin fragment or immunoglobulin fusion with G1 glycostructure comprising incubating with a galactosyltransferase, a sialyltransferase, a beta-1,4-galactosidase and a sialidase, whereby the galactosyltransferase is added in more than one aliquot during the incubating.
Claims8
-4. (canceled)
A method for producing an immunoglobulin or immunoglobulin fragment or fusion polypeptide, having a G1 glycostructure, comprising the following steps:
- incubating an affinity chromatography column eluate containing the immunoglobulin or immunoglobulin fragment or fusion polypeptide with a galactosyltransferase to obtain a galactosyltransferase reaction product,
- incubating the galactosyltransferase reaction product with a sialyltransferase to obtain a sialytransferase reaction product,
- incubating the sialyltransferase reaction product with a beta-1,4-galactosidase to obtain a beta-1,4-galactosidase reaction product,
- removing or inactivating the beta-1,4-galactosidase, and
- incubating the beta-1,4-galactosidase reaction product, in which the beta-1,4-galactosidase has been removed or inactivated, with a sialidase and thereby producing the immunoglobulin or immunoglobulin fragment or fusion polypeptide with the G1 glycostructure.
2 more elements
Dependencies are inferred from the claim text.