Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsYu Zhang, Junxin Duan, Ye Liu, Lan Tang
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Abstract
Disclosed are isolated polypeptides having cellobiohydrolase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. Furthermore, nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains are disclosed.
Claims15
1-18. (canceled)
An isolated polypeptide having cellobiohydrolase activity, selected from the group consisting of:
- (a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 4;
- or at least 89% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 3 or the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequence of SEQ ID NO:
- 3 or the cDNA sequence of SEQ ID NO:
- 1, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3 or the cDNA sequence thereof;
- or at least 89% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 4 or the mature polypeptide of SEQ ID NO:
- 2 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has cellobiohydrolase activity.
An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
- (a) a catalytic domain having at least 80% sequence identity to amino acids 106 to 469 of SEQ ID NO:
- 4 or at least 89% sequence identity to amino acids 44 to 393 of SEQ ID NO:
- 2;
- (b) a catalytic domain encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) nucleotides 400 to 1659 of SEQ ID NO:
- 3 or nucleotides 130 to 1376 of SEQ ID NO:
- 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a catalytic domain encoded by a polynucleotide having at least 80% sequence identity to the catalytic domain of SEQ ID NO:
- 3 or the cDNA sequence thereof;
- or a catalytic domain encoded by a polynucleotide having at least 89% sequence identity to the catalytic domain of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- (d) a variant of amino acids 106 to 469 of SEQ ID NO:
- 4 or amino acids 44 to 393 of SEQ ID NO:
- 2 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions;
- and
- (e) a fragment of the catalytic domain of (a), (b), (c), or (d) that has cellobiohydrolase activity.
An isolated polypeptide comprising a carbohydrate binding domain, wherein the carbohydrate binding domain is selected from the group consisting of:
- (a) a carbohydrate binding domain having at least 80% sequence identity to amino acids 24 to 63 of SEQ ID NO:
- 4;
- (b) a carbohydrate binding domain encoded by a polynucleotide that hybridizes under medium stringency conditions with (i) nucleotides 70 to 273 of SEQ ID NO:
- 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a carbohydrate binding domain encoded by a polynucleotide having at least 80% sequence identity to nucleotides 70 to 273 of SEQ ID NO:
- 3 or the cDNA sequence thereof;
- (d) a variant of amino acids 24 to 63 of SEQ ID NO:
- 4 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions;
- and
- (e) a fragment of (a), (b), (c), (d) or (e) that has cellulose binding activity.
Dependencies are inferred from the claim text.