Dynamic of sflt-1 or endoglin/plgf ratio as an indicator for imminent preeclampsia and/or hellp syndrome

A method for diagnosing whether a pregnant subject is at risk for developing preeclampsia within a short period of time comprising:

• a) determining the amounts of the biomarkers sFlt-1 or Endoglin and PIGF in a first and a second sample of said subject, wherein said first sample has been obtained prior to said second sample;
• b) calculating a first ratio from said amounts of sFlt-1 or Endoglin and PIGF determined in the first sample and a second ratio from said amounts of sFlt-1 or Endoglin and PIGF determined in the second sample;
• c) comparing the value of the said first and the said second ratio, whereby a subject being at risk for developing preeclampsia within a short period of time is diagnosed if the value of the second ratio is increased compared to the value of the first ratio by a factor of at least 3±20%.

A method for differentiating between a pregnant subject being at risk of developing early-onset-preeclampsia and a pregnant subject being not at risk of developing early-onset-preeclampsia comprising:

• a) determining the amounts of the biomarkers sFlt-1 or Endoglin and PIGF in a first and a second sample of said subject, wherein said first sample has been obtained prior to said second sample;
• b) calculating a first ratio from said amounts of sFlt-1 or Endoglin and PIGF determined in the first sample and a second ratio from said amounts of sFlt-1 or Endoglin and PIGF determined in the second sample;
• c) comparing the value of the said first and the said second ratio, whereby a subject being at risk for developing early-onset-preeclampsia is diagnosed if the value of the second ratio is increased compared to the value of the first ratio by a factor of at least about 3.