Compositions and methods for in vitro diagnostic tests including sulfonic acid
AssigneeSurModics, Inc.
InventorsGary Opperman, Wendy Nelson
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Abstract
The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.
Claims20
A composition for colorimetric analysis comprising
- an enzyme;
- a chromogenic reaction product;
- and
- a sulfonic acid that is a compound of Formula I:
- R—SO3H
- wherein R is methyl, ethyl, propyl, —NH2, or mixtures thereof.
A method of conducting colorimetric analysis comprising steps of:
- (a) providing a reaction composition comprising an enzyme and a chromogenic substrate;
- (b) allowing the reaction composition to form a chromogenic reaction product;
- (c) adding a sulfonic acid to the composition comprising the chromogenic reaction product, wherein the sulfonic acid is a compound of Formula I:
- R—SO3H
- wherein R is methyl, ethyl, propyl, —NH2, or mixtures thereof;
- and
- (d) after step (c), performing colorimetric analysis on the composition comprising the reaction product.
A kit for colorimetric analysis comprising;
- a chromogenic substrate capable of forming a chromogenic reaction product, and
- a sulfonic acid that is a compound of Formula I:
- R—SO3H
- wherein R is methyl, ethyl, propyl, —NH2, or mixtures thereof.
A method for stopping a reaction in a composition comprising a chromogenic reaction product, the method comprising a step of:
- adding a sulfonic acid to a reaction composition comprising an enzyme and a chromogenic reaction product, wherein the sulfonic acid is a compound of Formula I:
- R—SO3H
- wherein R is methyl, ethyl, propyl, —NH2, or mixtures thereof,
- wherein the sulfonic acid stops reaction in the composition.
Dependencies are inferred from the claim text.