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US 2014/0234866 A1Published application
Ex vivo flow cytometry method and device
AssigneePurdue Research Foundation
InventorsPhilip Stewart LOW, Wei HE, Sumith A. KULARATNE
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Abstract
The invention relates to a method for diagnosing a disease state mediated by pathogenic cells. The method comprises the steps of combining with an ex vivo patient sample a composition comprising a conjugate or complex of the general formula Ab-X wherein the group Ab comprises a ligand that binds to the pathogenic cells and the group X comprises an imaging agent, and detecting the pathogenic cells that express a receptor for the ligand using flow cytometry.
Claims22
-32. (canceled)
A method of detecting an imaging agent bound to pathogenic cells comprising the steps of:
- (i) combining 1) an ex vivo patient sample comprising the pathogenic cells collected from a patient, wherein the ex vivo patient sample is a body fluid of the patient, and 2) a composition comprising a conjugate of the formula
- Ab-X
- wherein X is the imaging agent, wherein the imaging agent emits a detectable signal, and
- wherein Ab comprises a prostate-specific membrane antigen binding ligand, and wherein the prostate-specific membrane antigen binding ligand binds to the prostate-specific membrane antigen on the pathogenic cells;
- and
- (ii) detecting, using flow cytometry, the signal from the imaging agent moiety of the conjugate wherein the conjugate is bound to the pathogenic cells.
3 more elements
Dependencies are inferred from the claim text.