Compositions and methods for toxigenicity testing
AssigneeCellsnap LLC
InventorsEric Arthur Johnson, Sabine Pellett, William Howard Tepp, Regina Clare Meyer Whitemarsh
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Abstract
The present invention relates to compositions and methods for testing agents (e.g., Clostridium botulinum neurotoxin (BoNT) detection and analysis). In particular, the present invention relates to the use of Human induced pluripotent stem (hiPS) derived cells for agent detection and analysis.
Claims23
1-21. (canceled)
A method of assaying a Clostridium botulinum neurotoxin (BoNT) for activity, comprising the steps of:
- a) contacting a human induced pluripotent stem (hiPS) derived neuronal cell with a composition comprising a BoNT;
- and
- b) assaying the BoNT for biological activity.
A method of assaying a Clostridium botulinum neurotoxin (BoNT) for activity, comprising the steps of:
- (a) contacting a human induced pluripotent stem (hiPS) derived neuronal cell with a composition comprising i) a BoNT and ii) a neutralizing antibody;
- and
- (b) assaying the BoNT for biological activity.
A method for determining an amount of biologically active Clostridium botulinum neurotoxin (BoNT) in a preparation, comprising the steps of:
- (a) contacting a human induced pluripotent stem (hiPS) derived neuronal cell with a sample of a preparation comprising biologically active BoNT;
- and
- (b) determining the amount of biologically active BoNT present in the preparation by assaying the sample for the biological activity of BoNT.
Dependencies are inferred from the claim text.