Recombinase Polymerase Amplification
AssigneeAlere San Diego, Inc.
InventorsOlaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
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Abstract
This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
Claims41
-187. (canceled)
A composition comprising:
- (1) at least one recombinase;
- (2) at least one polymerase;
- (3) a target nucleic acid;
- (4) a crowding agent;
- and
- (5) a first nucleic acid primer and optionally a second nucleic acid primer.
A kit comprising:
- (1) at least one recombinase;
- (2) at least one polymerase;
- and
- (3) a crowding agent.
A reagent mixture for nucleic acid amplification comprising:
- (1) at least one recombinase;
- (2) at least one polymerase;
- and
- (3) a crowding agent.
Dependencies are inferred from the claim text.