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US 2014/0234844 A1Published application
Hybridization compositions and methods using formamide
AssigneeDako Denmark A/S
InventorSteen Hauge Matthiesen
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Abstract
The invention provides methods and compositions for hybridizing at least one molecule to a target. The composition comprises at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.
Claims51
A hybridization composition comprising at least one nucleic acid sequence, formamide, and a hybridization solution, wherein the concentration of formamide is less than or equal to 25%.
A method of hybridizing nucleic acid sequences comprising:
- providing a first nucleic acid sequence,
- providing a second nucleic acid sequence,
- providing a hybridization composition comprising formamide, and
- combining the first and the second nucleic acid sequence and the hybridization composition for at least a time period sufficient to hybridize the first and second nucleic acid sequences,
- wherein the concentration of formamide is less than or equal to 25%.
2 more elements
A method of hybridizing nucleic acid sequences comprising:
- providing a first nucleic acid sequence in an in situ biological sample, and
- applying a hybridization composition comprising a second nucleic acid sequence and formamide to said first nucleic acid sequence for at least a time period sufficient to hybridize the first and second nucleic acid sequences,
- wherein the formamide is present in a concentration less than or equal to 25%.
(canceled)
Dependencies are inferred from the claim text.