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US 2014/0234841 A1Published application
Compositions, methods and kits to detect dicer gene mutations
AssigneesChildren's Hospital and Clinics of Minnesota, Washington University in St. Louis
InventorsAshley D. Hill, Paul Goodfellow, John R. Priest, Yoav Messinger
Life of this patent
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Abstract
In one aspect, the disclosure provides isolated nucleic acids, polypeptides, primers, and probes for the detection of mutations in a nucleic acid sequence for a DICER1 polypeptide.
Claims13
A kit comprising a nucleic acid selected from the group consisting of:
- a primer that amplifies a portion of an isolated nucleic acid that encodes a portion of a DICER1 polypeptide or that comprises a portion of the DICER1 gene, wherein the nucleic acid comprises a mutation in the isolated nucleic acid sequence as compared to a corresponding sequence in a reference nucleic acid encoding a DICER polypeptide having a sequence of SEQ ID NO:1, and wherein the mutation in the DICER1 polypeptide or gene decreases RNAse function of DICER1 polypeptide;
- a probe that hybridizes to a portion of the nucleic acid that encodes a portion of a DICER1 polypeptide or that comprises a portion of the DICER1 gene, wherein the nucleic acid comprises a mutation in the isolated nucleic acid sequence as compared to a corresponding sequence in a reference nucleic acid encoding a DICER polypeptide having a sequence of SEQ ID NO:1, and wherein the mutation in the DICER1 polypeptide or gene decreases RNAse function of DICER1 polypeptide;
- and combinations thereof.
Dependencies are inferred from the claim text.