Buttiauxella sp. phytase variants
AssigneeDanisco US Inc.
InventorsOliver Kensch, Klaus Schulze Pellengahr, Birgitta Leuthner, Jayarama K. Shetty, Michael Pepsin, Soren Dalsgaard, Mai Faurschou Isaksen
Life of this patent
Estimating the term…
Abstract
Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.
Claims16
1-15. (canceled)
A phytase variant having phytase activity and an amino acid sequence that varies from the amino acid sequence of wild type Buttiauxella sp. phytase (SEQ ID NO:
- 1), wherein the amino acid sequence of the phytase variant comprises at least one variation as compared with SEQ ID NO:
- 1, and wherein the phytase sequence variation comprises
- a) N37Y, S75P, A89T, D92A, T134I, H160R, F164E, T171V, T176K, A178P, S188P, G192A, K198R, K207E, A209S, S248L, Q256Y, A261E, N270K, A374P
- b) N37Y, G77S, A89T, D92A, T134I, H160R, F164E, T171V, T176K, A178P, S188P, G192A, K198R, K207E, A209S, S248L, Q256Y, A261E, N270K, A374P
- c) N37Y, S75P, Q76R, A89T, D92A, T134I, H160R, F164E, T171I, T176K, A178P, S188P, G192A, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, A374P
- d) N37Y, A89T, D92A, T134I, F164E, T171V, T176K, A178P, G192A, K207E, A209S, A235V, S248L, Q256P, A261E, N270K, A374P
- e) S75P, Q76R, A89T, D92A, T134I, H160R, F164E, T171I, T176K, A178P, S188P, G192A, K207E, A209S, S248L, Q256Y, A261E, N270K, A374P
- f) N37Y, Q76R, A89T, D92A, T134I, H160R, F164E, T171I, T176K, A178P, S188P, G192A, K207E, A209S, S248L, Q256Y, A261E, N270K, A374P
- g) N37Y, Q76R, A89T, D92A, T134I, F164S, T171V, T176K, A178P, S188P, G192A, K207E, A209S, A235V, S248L, Q256A, A261E, N270K, A374P
- h) S75P, A89T, D92A, T134I, F164E, T171V, T176K, A178P, S188P, G192A, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, A374P
- i) S75P, Q76R, A89T, D92A, T134I, H160R, F164E, T171V, T176K, A178P, S188P, G192A, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, P367L, A374P
- j) N37Y, A89T, D92A, T134I, F164E, T171I, T176K, A178P, G192A, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, A374P
- k) N37Y, Q76R, A89T, D92A, T134I, F164E, T171V, T176K, A178P, G192A, K207E, A209S, S248L, Q256Y, A261E, N270K, A374P
- l) N37Y, Q76R, A89T, D92A, T134I, F164E, T171V, T176K, A178P, G192A, K207E, A209S, S248L, Q256A, A261E, N270K, A374P
- m) N37Y, S75P, Q76R, A89T, D92A, T134I, F164E, T171V, T176K, A178P, K207E, A209S, A235V, S248L, Q256A, A261E, N270K, A374P
- n) N37Y, S75P, A89T, D92A, T134I, H160R, F164E, T171V, T176K, A178P, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, A374P
- o) N37Y, A89T, D92A, T134I, H160R, F164S, T171I, T176K, A178P, S188P, G192A, K207E, A209S, A235V, S248L, Q256E, A261E, N270K, A374P
- p) A89T, D92A, T134I, H160R, F164E, T171V, T176K, A178P, G192A, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, A374P
- q) N37Y, S75P, A89T, D92A, T134I, H160R, F164S, T171V, T176K, A178P, S188P, K207E, A209S, S248L, Q256H, A261E, N270K, A374P
- r) N37Y, S75P, A89T, D92A, T134I, F164S, T171V, T176K, A178P, S188P, G192A, K207E, A209S, S248L, Q256A, A261E, N270K, A374P
- s) S75P, Q76R, A89T, D92A, T134I, H160R, F164E, T171V, T176K, A178P, G192A, K207E, A209S, S248L, Q256A, A261E, N270K, A374P;
- or
- t) N37Y, Q76R, A89T, D92A, T134I, H160R, F164S, T171V, T176K, A178P, G192A, K207E, A209S, A235V, S248L, Q256Y, A261E, N270K, A374P.
A phytase variant having phytase activity and an amino acid sequence that varies from the amino acid sequence of the wild type Buttiauxella sp. phytase (SEQ ID NO:
- 1), wherein the amino acid sequence of the phytase variant comprises at least one variation as compared with SEQ ID NO:
- 1, and wherein the variant has a variation at at least any one of the following positions:
- 70, 193, 197, 221 and 407.
21-26. (canceled)
35-57. (canceled)
Dependencies are inferred from the claim text.