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US 2014/0234479 A1Published application
Microalgal flour
AssigneeCorbion Biotech, Inc.
InventorsLeslie Norris, John Piechocki, Enrique Baliu, Ruchir Desai, Marie Le Ruyet, Samuel Patinier, Damien Passé, Amandine Druon
Life of this patent
Estimating the term…
Abstract
The present invention relates to microalgal food products with acceptable sensory characteristics and methods of producing the food products. The flour can be produced by cultivating microalgal cells of a strain of Chlorella protothecoides under conditions of acceptable pH and dissolved oxygen to produce a desired amount of lipid. The microalgal cells can be lysed, heat-treated, washed and dried to produce a microalgal flour that can be incorporated into a variety of products.
Claims36
A microalgal flour suitable for use in food, the flour comprising microalgal cells of Chlorophyta and characterized by a flavor descriptor falling within an ellipsoid in a flavor-description space having dimensions of PC1, PC2 and PC3, the flavor descriptor produced by using SPME and/or SBSE analysis to determine concentrations of the following compounds:
- Dimethyl.sulfide
- 2.3.Butanedione
- Butanal
- Propanal..2.methyl.
- Furan..3.methyl.
- Ethyl.Acetate
- 2.Butenal...E..
- Butanal..3.methyl.
- 1.Butanol
- Butanal..2.methyl.
- Thiophene
- 1.Penten.3.ol
- 1.Penten.3.one
- 2.Pentanone
- 2.3.Pentanedione
- Pentanal
- Furan..2.ethyl.
- Thiazole
- 3.Penten.2.one
- Disulfide..dimethyl
- 2.Pentenal...E..
- Pyrrole
- Oxazole..4.5.dimethyl.
- 2.Penten.1.ol...Z..
- Thiophene..3.methyl.
- Hexanal
- 4.Methylthiazole
- Pyrazine..methyl.
- Furfural
- Oxazole..trimethyl.
- Butanoic.acid..3.methyl.
- Butanoic.acid..2.methyl.
- 2.Hexenal
- 1.Hexanol
- 4.Heptanone
- Pyridine..2.6.dimethyl.
- Thiazole..2.4.dimethyl.
- 3.Heptanone
- 2.Heptanone
- 3.Heptanol
- Heptanal
- Methional
- Pyrazine..2.5.dimethyl.
- Pyrazine..2.6.dimethyl.
- Pyrazine..ethyl.
- Pyrazine..2.3.dimethyl.
- Pyrazine..ethenyl.
- Thiazole..4.5.dimethyl.
- 2.Heptanone..6.methyl.
- Hexanal..2.ethyl.
- 2.Heptenal...Z..
- 5.Nonen.2.one
- 2.Furancarboxaldehyde..5.methyl
- Benzaldehyde
- hexanoic.acid
- 1.Octen.3.ol
- Dimethyl.trisulfide
- 2.5.Octanedione
- 5.Hepten.2.one..6.methyl.
- Furan..2.pentyl.
- 2.4.Heptadienal...E.E..
- Pyrazine..2.ethyl.6.methyl.
- Octanal
- Pyrazine..trimethyl.
- Pyrazine..2.ethyl.3.methyl.
- 2.4.Heptadienal...E.E...1
- Pyrazine..2.ethenyl.6.methyl.
- 1.Hexanol..2.ethyl.
- 3.Octen.2.one...E..
- 2H.Pyran.2.one..5.6.dihydro.
- Benzeneacetaldehyde
- 3.5.Octadien.2.one...E.E..
- Acetophenone
- 1.Decen.3.one
- Pyrazine..3.ethyl.2.5.dimethyl.
- Pyrazine..tetramethyl.
- 5.Methyl.2.thiophenecarboxaldehyde
- g.Heptalactone
- Linalool
- Nonanal
- Thymol
- Phenylethyl.Alcohol
- 2.3.5.Trimethyl.6.ethylpyrazine.
- Acetic.acid..phenylmethyl.ester
- Safranal
- 2.Decenal...E..
- g.octalacone
- o.Amino.acetophenone
- 2.4.Decadienal
- g.Nonlactone
- Ionone
- Geranyl.acetone
- Ionene
- g.Nonlactone.1
- 2.4.Nonadienal...E.E..
- 2.4.Decadienal.1
- g.Heptalactone.1
- Ionone.1
- Geranyl.acetone.1
- a.Ionone
- Peach.lactone.g.undecalactone
- d.Decalactone
- cis.Geranylacetone
- d.dodecalactone..6.Nonyl.6.valeralactone.
- d.Undecalactone
- relative to an internal standard,
- the ellipsoid defined by the equation Ax2+Bxy+Cy2+Dx+Ey+F=0 and parameterized according to the following table:
- X
- Y
- Dimen-
- Dimen-
- sion
- sion
- A
- B
- C
- D
- E
- F
- PC1
- PC2
- 0.003481467
- −0.000366174
- 3.79437E−05
- −0.000628924
- 4.27301E−05
- 1.51548E−05
- PC1
- PC3
- 0.001734328
- 0.000286969
- 1.89401E−05
- −0.000318201
- −2.8099E−05
- 1.12003E−05
- PC2
- PC3
- 0.356218856
- 0.289219807
- 0.356936631
- 0.085191149
- −0.040237159
- −0.13812915
- wherein, the falling within the ellipsoid is determined by the procedure of:
- for each compound, determining relative concentrations;
- for each compound, subtracting center factors according to the table below;
- for each compound, dividing by the scale factors according to the table below;
- taking the dot product of the scaled and centered data to yield values for PC1, PC2 and PC3;
- and
- determining if the flavor descriptor defined by PC1, PC2 and PC3 falls within the ellipsoid:
- Chemical
- Center
- Scale
- PC1
- PC2
- PC3
- Dimethyl.sulfide
- 15.04166667
- 52.10586179
- 0.007602386
- −0.154648539
- 0.13795639
- 2.3.Butanedione
- 573.4583333
- 687.3035077
- −0.053406645
- 0.116238372
- 0.138457708
- Butanal
- 165.0833333
- 291.8766733
- −0.061200873
- 0.021748265
- −0.154199309
- Propanal..2.methyl.
- 294.25
- 321.9922006
- −0.02479716
- −0.203551061
- 0.142079295
- Furan..3.methyl.
- 254.0833333
- 364.0905752
- −0.139050167
- 0.053488926
- −0.040009249
- Ethyl.Acetate
- 1534.958333
- 721.2414001
- 0.023033335
- 0.078632968
- 0.149060426
- 2.Butenal...E..
- 56.95833333
- 67.74264748
- 0.034598984
- −0.007869304
- −0.228855217
- Butanal..3.methyl.
- 2368.958333
- 3305.894731
- −0.015854973
- −0.209996041
- 0.152553963
- 1.Butanol
- 236.75
- 723.0508438
- 0.01482126
- 0.147080874
- 0.120323863
- Butanal..2.methyl.
- 858.0416667
- 1132.843254
- −0.069765232
- −0.186610612
- 0.143374765
- Thiophene
- 0.708333333
- 2.453738644
- −0.145349572
- 0.003673658
- −0.010721336
- 1.Penten.3.ol
- 111.2916667
- 123.2715883
- −0.105910877
- 0.059069801
- −0.020890092
- 1.Penten.3.one
- 10.625
- 18.86570361
- 0.029319785
- −0.055925743
- −0.186580083
- 2.Pentanone
- 429.875
- 520.4705967
- 0.018948769
- −0.168215403
- 0.184382338
- 2.3.Pentanedione
- 392.625
- 359.8726495
- 0.037715762
- −0.074625863
- −0.010390137
- Pentanal
- 5315.166667
- 4258.727501
- −0.05954475
- −0.05904769
- −0.130129097
- Furan..2.ethyl.
- 32.75
- 24.43590875
- −0.008414663
- −0.076099651
- −0.014167153
- Thiazole
- 70.16666667
- 199.0549642
- −0.142882049
- −0.031332244
- 0.020544457
- 3.Penten.2.one
- 442.125
- 470.5612763
- 0.036579138
- −0.118623927
- 0.193220234
- Disulfide..dimethyl
- 77.45833333
- 105.2821875
- 0.007660621
- 0.076749927
- −0.030508003
- 2.Pentenal...E..
- 116.7083333
- 200.60312
- 0.029036734
- 0.005658787
- −0.063353931
- Pyrrole
- 12.29166667
- 41.79846579
- −0.145424967
- 0.001008736
- −0.008354639
- Oxazole..4.5.dimethyl.
- 15.83333333
- 54.84827557
- −0.145349572
- 0.003673658
- −0.010721336
- 2.Penten.1.ol...Z..
- 45.25
- 118.0232065
- −0.141807908
- −0.022407562
- −0.007205637
- Thiophene..3.methyl.
- 108.5416667
- 279.7959856
- 0.006693629
- 0.144512146
- 0.116341706
- Hexanal
- 26189.95833
- 17886.61913
- 0.023290612
- 0.064196972
- −0.162118696
- 4.Methylthiazole
- 1.958333333
- 6.783865663
- −0.145349572
- 0.003673658
- −0.010721336
- Pyrazine..methyl.
- 135.2083333
- 326.6405766
- −0.138842567
- −0.055435505
- 0.03372617
- Furfural
- 34.5
- 119.5115057
- −0.145349572
- 0.003673658
- −0.010721336
- Oxazole..trimethyl.
- 64
- 221.7025034
- −0.145349572
- 0.003673658
- −0.010721336
- Butanoic.acid..3.methyl.
- 58.58333333
- 202.9386196
- −0.145349572
- 0.003673658
- −0.010721336
- Butanoic.acid..2.methyl.
- 3.833333333
- 13.27905619
- −0.145349572
- 0.003673658
- −0.010721336
- 2.Hexenal
- 25.58333333
- 50.09710268
- 0.027469429
- −0.052249399
- −0.23615517
- 1.Hexanol
- 106.1666667
- 155.9474465
- 0.031207096
- 0.198558566
- 0.011983686
- 4.Heptanone
- 360.5833333
- 577.8576749
- −0.003575779
- −0.135096305
- 0.010019679
- Pyridine..2.6.dimethyl.
- 2.958333333
- 10.24796728
- −0.145349572
- 0.003673658
- −0.010721336
- Thiazole..2.4.dimethyl.
- 15.58333333
- 53.98225017
- −0.145349572
- 0.003673658
- −0.010721336
- 3.Heptanone
- 111.625
- 94.41016052
- 0.021607662
- −0.18444557
- −0.171655667
- 2.Heptanone
- 380.875
- 288.460973
- −0.097016748
- −0.058868123
- 0.015417076
- 3.Heptanol
- 1193.041667
- 1008.348074
- 0.023029974
- −0.205456135
- −0.111328282
- Heptanal
- 1396.791667
- 920.0702903
- −0.113307135
- 0.141565621
- −0.025917554
- Methional
- 79.625
- 148.3023823
- −0.110012922
- −0.130400953
- 0.093977633
- Pyrazine..2.5.dimethyl.
- 3.333333333
- 7.857634774
- 0.020631611
- −0.116950274
- −0.004255769
- Pyrazine..2.6.dimethyl.
- 178.2083333
- 574.8013672
- −0.145388496
- −0.007146465
- −0.001098366
- Pyrazine..ethyl.
- 15.95833333
- 53.8796885
- −0.145442956
- −0.0000479
- −0.007415618
- Pyrazine..2.3.dimethyl.
- 439.2083333
- 1498.775644
- −0.145413873
- 0.001518449
- −0.008807482
- Pyrazine..ethenyl.
- 1.416666667
- 4.907477288
- −0.145349572
- 0.003673658
- −0.010721336
- Thiazole..4.5.dimethyl.
- 3.583333333
- 12.41303079
- −0.145349572
- 0.003673658
- −0.010721336
- 2.Heptanone..6.methyl.
- 53.75
- 186.1954618
- −0.145349572
- 0.003673658
- −0.010721336
- Hexanal..2.ethyl.
- 78.41666667
- 124.9672381
- 0.018460956
- −0.027007294
- −0.179937424
- 2.Heptenal...Z..
- 645.25
- 937.3877266
- 0.021607084
- −0.093800543
- −0.190591625
- 5.Nonen.2.one
- 13.33333333
- 46.18802154
- −0.145349572
- 0.003673658
- −0.010721336
- 2.Furancarboxaldehyde..5.
- 21.25
- 40.57288615
- 0.019206035
- −0.109620677
- 0.175448337
- methyl
- Benzaldehyde
- 872.875
- 1358.161493
- −0.142431906
- 0.046335544
- 0.024776943
- hexanoic.acid
- 176.25
- 216.4210438
- −0.001128927
- 0.064879481
- −0.016090326
- 1.Octen.3.ol
- 369.6666667
- 350.9919277
- −0.090672545
- −0.045064295
- −0.135474824
- Dimethyl.trisulfide
- 14.33333333
- 21.56315601
- 0.028899179
- −0.064852089
- −0.150867075
- 2.5.Octanedione
- 23.95833333
- 44.27674248
- 0.028988465
- −0.07590479
- −0.093752193
- 5.Hepten.2.one..6.methyl.
- 1503.833333
- 4827.634134
- −0.145266246
- 0.005470194
- −0.014175912
- Furan..2.pentyl.
- 633
- 967.4016276
- −0.078384616
- 0.167579691
- −0.035610073
- 2.4.Heptadienal...E.E..
- 20.83333333
- 43.16371231
- 0.024003523
- −0.071588186
- −0.145038829
- Pyrazine..2.ethyl.6.methyl.
- 21
- 72.74613392
- −0.145349572
- 0.003673658
- −0.010721336
- Octanal
- 1243.041667
- 897.5365644
- 0.063418428
- 0.197764097
- −0.01447548
- Pyrazine..trimethyl.
- 348.6666667
- 1051.439497
- −0.144625394
- −0.018888681
- 0.009357594
- Pyrazine..2.ethyl.3.methyl.
- 87.33333333
- 302.5315411
- −0.145349572
- 0.003673658
- −0.010721336
- 2.4.Heptadienal...E.E...1
- 26.33333333
- 40.42070427
- 0.033749609
- −0.100784032
- −0.199828071
- Pyrazine..2.ethenyl.6.methyl.
- 5.541666667
- 19.19689645
- −0.145349572
- 0.003673658
- −0.010721336
- 1.Hexanol..2.ethyl.
- 5684.541667
- 5078.453328
- 0.015454406
- −0.147033095
- −0.173896762
- 3.Octen.2.one...E..
- 196.375
- 462.4334412
- 0.022433793
- −0.027668713
- −0.141800019
- X2H.Pyran.2.one..5.6.dihydro.
- 683.3333333
- 845.025291
- 0.040235145
- 0.008083104
- −0.001975331
- Benzeneacetaldehyde
- 31.83333333
- 60.74811383
- 0.01141478
- −0.200551415
- 0.147671091
- 3.5.Octadien.2.one...E.E..
- 455.125
- 426.6112306
- 0.024307307
- 0.191552198
- −0.040535191
- Acetophenone
- 42.375
- 56.41088104
- 0.034819826
- 0.112028714
- 0.067831917
- 1.Decen.3.one
- 3.125
- 9.100761706
- 0.014871492
- −0.007143686
- 0.067973089
- Pyrazine..3.ethyl.2.5.dimethyl.
- 50.75
- 174.3908228
- −0.145387371
- 0.002524067
- −0.009700663
- Pyrazine..tetramethyl.
- 951.4583333
- 3113.918129
- −0.145437121
- −0.00391206
- −0.005426362
- 5.Methyl.2.thiophenecarboxaldehyde
- 57.375
- 198.7528302
- −0.145349572
- 0.003673658
- −0.010721336
- g.Heptalactone
- 2
- 6.92820323
- 0.012980337
- 0.140814237
- 0.118375646
- Linalool
- 9.833333333
- 34.06366588
- −0.145349572
- 0.003673658
- −0.010721336
- Nonanal
- 1528.416667
- 1335.036088
- 0.053558189
- 0.198785653
- −0.109289305
- Thymol
- 160.5833333
- 556.2769844
- −0.145349572
- 0.003673658
- −0.010721336
- Phenylethyl.Alcohol
- 135.9583333
- 416.085189
- −0.145061726
- −0.01428243
- 0.003239013
- 2.3.5.Trimethyl.6.ethylpyrazine.
- 208.7083333
- 718.7459552
- −0.145377878
- 0.002836895
- −0.00997845
- Acetic.acid..phenylmethyl.
- 213.875
- 205.6043337
- 0.045438482
- 0.114758954
- 0.153953593
- ester
- Safranal
- 47.29166667
- 163.8231389
- −0.145349572
- 0.003673658
- −0.010721336
- 2.Decenal...E..
- 55.04166667
- 78.60616976
- 0.034351801
- −0.012969523
- −0.21493625
- g.octalacone
- 10.625
- 28.57933535
- 0.016392036
- 0.14295305
- 0.096452129
- o.Amino.acetophenone
- 15.5
- 32.17070943
- 0.022315438
- 0.204041622
- 0.018370134
- 2.4.Decadienal
- 9.416666667
- 24.16781606
- 0.0179089
- 0.169004115
- −0.038947428
- g.Nonlactone
- 13.5
- 40.20345982
- 0.01493418
- 0.189230257
- 0.033376822
- Ionone
- 101.3333333
- 351.0289637
- −0.145349572
- 0.003673658
- −0.010721336
- Geranyl.acetone
- 652.75
- 2137.396627
- −0.145423518
- −0.002004031
- −0.008551463
- Ionene
- 159.7916667
- 553.5345706
- −0.145349572
- 0.003673658
- −0.010721336
- g.Nonlactone.1
- 6.58755
- 22.81994259
- 0.016371012
- −0.075372449
- −0.049632645
- 2.4.Nonadienal...E.E..
- 18.07305674
- 30.64101284
- 0.031363408
- −0.023742328
- −0.174506137
- 2.4.Decadienal.1
- 50.4716275
- 85.11825112
- 0.029518821
- 0.094376773
- −0.171060695
- g.Heptalactone.1
- 17.25928968
- 42.07909242
- 0.017750131
- 0.158720982
- −0.019846703
- Ionone.1
- 199.0162875
- 689.4126429
- −0.145349572
- 0.003673658
- −0.010721336
- Geranyl.acetone.1
- 880.2922516
- 3049.421811
- −0.145349572
- 0.003673658
- −0.010721336
- a.Ionone
- 335.0475951
- 1160.638915
- −0.145349572
- 0.003673658
- −0.010721336
- Peach.lactone.g.undecalactone
- 72.77877498
- 34.06000193
- 0.097029409
- −0.071461906
- 0.084434422
- d.Decalactone
- 85.57314465
- 106.5309321
- 0.034674859
- −0.18805394
- 0.077061807
- cis.Geranylacetone
- 5.9584
- 20.64050306
- 0.011926134
- 0.016184168
- −0.063393798
- d.dodecalactone..δ.Nonyl.
- 1400.955104
- 491.4817796
- 0.130734715
- −0.059212775
- 0.033318423
- δ.valeralactone.
- d.Undecalactone
- 6472.792302
- 6394.323609
- 0.051826724
- −0.042456918
- −0.131176612.
786 more elements
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A microalgal flour suitable for use in food, the flour comprising microalgal cells of Chlorophyta, wherein analysis by SPME according to Example 4 and/or SBSE according to Example 5 to determine concentrations of the compounds of Example 6 relative to an internal standard, followed by analysis according to the procedure of Example 9 produces a flavor descriptor that falls within the ellipsoid of Example 8 defining 3 standard deviations relative to the positive flavor cluster corresponding to the closed circles in the graph of FIG. 2.
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Dependencies are inferred from the claim text.