Methods and products for in vivo enzyme profiling
AssigneeMassachusetts Institute of Technology
InventorsSangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
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Abstract
The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
Claims35
1-50. (canceled)
A method comprising
- collecting a urine sample from a subject suspected of having a disorder or condition, wherein the subject has been administered a pro-diagnostic reagent, the pro-diagnostic reagent comprising a carrier domain linked to an enzyme susceptible domain which is linked to a signature molecule;
- and,
- subjecting the urine sample to a multiplex analysis method in order to detect the presence of the signature molecule, and wherein the presence or absence of the signature molecule in the biological sample is indicative of the disorder or condition within the subject.
A reagent comprising
- a carrier domain, wherein the carrier domain is a particle and is greater than 5 nm in size;
- an enzyme susceptible domain linked to the carrier domain;
- and,
- a signature molecule linked to the enzyme susceptible domain, wherein the signature molecule is a peptide or nucleic acid.
A reagent comprising an implantable microdelivery device housing a modular structure having a carrier domain linked to a signature producing domain.
A reagent comprising
- a carrier domain having a plurality of enzyme susceptible domains linked to the carrier domain wherein each enzyme susceptible domain is linked to a non-fluorescent signature molecule.
A composition comprising
- a plurality of pro-diagnostic reagents comprising a carrier domain, an enzyme susceptible domain linked to the carrier domain;
- and, a non-fluorescent signature molecule linked to the enzyme susceptible domain.
A kit comprising
- a container housing a pro-diagnostic reagent, wherein the pro-diagnostic reagent comprises modular structure having a carrier domain linked to a signature producing domain;
- and,
- instructions for administering the pro-diagnostic reagent to a subject and for analyzing a signature molecule generated by administration of the pro-diagnostic reagent in a biological sample of the subject.
84-88. (canceled)
Dependencies are inferred from the claim text.