Prame purification
AssigneeGlaxosmithkline Biologicals SA
InventorsOlivier C Germay, Stephane Andre Godart, Pol Guy Harvengt, Amina Laanan, Olivier Patrick Le Bussy, Dominique Ingrid Lemoine, Leonard Dode
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Abstract
Methods and processes for the purification of PRAME are provided. In particular, methods for reducing the aggregation of PRAME during a diluent exchange from diluent A to diluent B comprising: (i) adding a polyanionic compound to diluent A prior to or contemporaneously with the exchange; and (ii) exchanging protein from diluent A to diluent B are provided. Compositions produced by the method are also provided.
Claims28
1-28. (canceled)
A method for reducing the aggregation of a protein during a diluent exchange from diluent A to diluent B comprising:
- (i) adding a polyanionic compound to diluent A prior to or contemporaneously with the exchange;
- and
- (ii) exchanging protein from diluent A to diluent B, wherein the protein is PRAME.
A composition comprising PRAME and an oligonucleotide, wherein PRAME has a particle size of between 10-30 nm.
A process for producing a pharmaceutically acceptable PRAME composition comprising the steps of:
- (a) carrying out a diluent exchange of PRAME from diluent A to diluent B, wherein a polyanionic compound is added to diluent A or diluent B prior to or during the diluent exchange;
- and
- (b) obtaining diluent B comprising PRAME.
Dependencies are inferred from the claim text.