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AssigneeActogenix N.V.
InventorsLothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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The present invention relates to a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, with increased stress resistance and/or improved manufacturing, processing and/or storage characteristics. In particular, the invention relates to a gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further lack cellobiose-specific PTS system IIC component (ptcC) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.
A composition comprising a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, lacking trehalose 6-phosphate phosphorylase (TrePP) activity for use as a medicament, preferably wherein the gene encoding endogenous TrePP has been partially or completely deleted, disrupted or inactivated such as being incapable of producing functional trePP gene product, in combination with a pharmaceutical carrier.
A food additive, probiotic composition, or a starter culture comprising a gram positive bacterium lacking TrePP activity, preferably wherein the gene encoding endogenous TrePP has been partially or completely deleted, disrupted or inactivated such as being incapable of producing functional trePP gene product.
(canceled)
A method for internally accumulating trehalose in a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, comprising propagating the gram positive bacterium, wherein the gram positive bacterium lacks TrePP activity, preferably wherein the gene encoding endogenous TrePP has been partially or completely deleted, disrupted or inactivated such as being incapable of producing functional TrePP gene product, in a medium comprising a substrate material capable of being fermented by said gram positive bacterium, whereby trehalose is accumulated in the gram positive bacterium.
26-29. (canceled)
Dependencies are inferred from the claim text.