US 2014/0234355 A1 · INFECTIOUS CLONES OF TORQUE TENO VIRUS

Abstract

The present invention is directed to novel nucleotide and amino acid sequences of Torque teno virus (“TTV”), including novel genotypes thereof, all of which are useful in the preparation of vaccines for treating and preventing diseases in swine and other animals. Vaccines provided according to the practice of the invention are effective against multiple swine TTV genotypes and isolates. Diagnostic and therapeutic polyclonal and monoclonal antibodies are also a feature of the present invention, as are infectious clones useful in the propagation of the virus and in the preparation of vaccines. Particularly important aspects of the invention include vaccines that provide TTV ORF1 protein, or peptide fragments thereof, as antigen.

Claims14

Dependent claims

An isolated polynucleotide sequence that comprises a polynucleotide selected from the group consisting of:

(a1) the DNA of genotype 2 sequence TTV13 (SEQ ID NO:
1);
the DNA genotype 2 sequence TTV10 (SEQ ID NO:
2);
or a fragment thereof than encodes the TTV capsid protein or a fragment of said protein;
(a2) the DNA of a genotype 1 sequence selected from the group consisting of ttvg1-7 (SEQ ID NO:
4), ttvGT1-17 (SEQ ID NO:
5), ttvGT1-21 (SEQ ID NO:
6), ttvgt1-27 (SEQ ID NO:
3), and ttvgt1-178 (SEQ ID NO:
7) or a fragment thereof than encodes the TTV capsid protein or a fragment of said protein;
(b) the complement of any sequence in (a);
(c) a polynucleotide that hybridizes with a sequence of (a) or (b) under stringent conditions defined as hybriding to filter bound DNA in 0.5M NaHPO4, 7% SDS, 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS at 68° C.;
(d) a polynucleotide that is at least 70% identical to the polynucleotide of (a) or (b);
(e) a polynucleotide that is at least 80% identical to the polynucleotide of (a) or (b);
(f) a polynucleotide that is at least 90% identical to the polynucleotide of (a) or (b);
and
(g) a polynucleotide that is at least 95% identical to the polynucleotide of or (b).

15 more elements

Dependencies are inferred from the claim text.

Infectious clones of torque teno virus

Life of this patent

Infectious clones of torque teno virus

Life of this patent

Abstract

Claims14