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AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Serv
InventorUdai S. Kammula
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The invention provides a method of obtaining a population of antigen-specific T cells from peripheral blood of a host. An embodiment of the method of the invention comprises (i) dividing PBMCs from peripheral blood of a host into more than one sub-population; (ii) contacting the PBMCs with an antigen and IL-2; (iii) obtaining a sample of PBMCs from each sub-population; (iv) identifying an antigen-reactive sub-population by determining by high throughput quantitative PCR the expression of a factor produced by the PBMCs of each sample; (v) dividing the antigen-reactive sub-population into microcultures; (vi) identifying the antigen-reactive microculture; and (vii) expanding the microculture, thereby obtaining a population of T cells specific for the antigen. The invention also provides a population of T cells obtained by the inventive method, a pharmaceutical composition comprising the same, and a method of treating a disease in a host using the pharmaceutical composition. Related isolating and screening methods are further provided.
A clinical grade population of antigen-specific T cells obtained by a method comprising:
(canceled)
A method of isolating antigen-specific T cells from peripheral blood of a host, comprising:
A method of screening candidate cancer antigen epitopes, comprising:
Dependencies are inferred from the claim text.