Diagnostic assay for tissue transplantation status
AssigneeMuroch Children Research Institute
InventorsHoward Robert Slater, Damien Luis Bruno, Devika Ganesamoorthy
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Abstract
The present disclosure relates generally to tissue, including organ, transplantation. Taught herein is a genetic-based diagnostic assay to ascertain the status of a tissue transplantation procedure in a recipient based on a characterization of circulating or other cell free nucleic acid material. The instant disclosure enables kits, primers and protocols for ascertaining the status of a transplantation procedure.
Claims27
A method for determining the status of a donor tissue implanted into a recipient, said method comprising screening a sample from the recipient for the presence or absence of circulatory nucleic acids which have a copy number variant (CNV) polymorphism which indicates they are donor-derived nucleic acids wherein the presence of donor nucleic acids is indicative of cellular damage of the transplanted tissue and the absence of donor nucleic acids or a level of donor nucleic acids relative to a control is indicative of no cellular damage or cellular damage to an acceptable level.
A transplantation protocol for transplanting tissue from a donor to a recipient, said protocol comprising transplanting the tissue and then monitoring a sample from the recipient for the presence of nucleic acids which are not the recipients wherein if donor nucleic acids are detected, the recipient is subjected to immunosuppression therapy or if the patient is on immunosuppression therapy, the therapy is altered.
-29. (canceled)
Dependencies are inferred from the claim text.